近年来，已经开发了几种纳米载体合成方法。在癌症治疗中，智能纳米载体的使用引起了人们的兴趣。智能纳米载体对其环境做出反应，并可以在内部或外部刺激的作用下以受控方式释放其货物。在这项工作中，我们报告了一种气溶胶辅助方法的开发，用于合成负载姜黄素的壳聚糖/藻酸盐基聚合物纳米载体（CurNCs）。使用定制的多雾化器系统来合成 CurNC。开发的系统包括三个主要部分：喷雾器、电加热通道和收集器。使用气动多雾化器将姜黄素和壳聚糖溶液喷入电加热器隧道中以形成壳聚糖-姜黄素组件。然后，将气溶胶导入含有海藻酸钠和三聚磷酸盐水溶液的收集剂溶液中进一步交联。使用 TEM、DLS 和 FTIR 技术对合成的 CurNC 进行表征。纳米颗粒的 TEM 尺寸为 8.62±2.25 nm。释放实验表明，纳米载体对环境 pH 值敏感，因为与生理 pH 值相比，在酸性 pH 值（癌组织的情况）下释放更多的姜黄素。纳米载体的姜黄素含量为77.27 mg g-1，载药率为62%。针对 MCF7 乳腺癌细胞系评估了合成纳米载体的体外细胞毒性。 CurNC 和姜黄素的 IC50 浓度分别为 14.86 和 16.45 mg mL-1。结果表明，虽然空纳米载体表现出不显着的细胞毒性，但 CurNC 会影响细胞培养物并导致细胞长期死亡。总体而言，pH响应性姜黄素聚合物纳米载体是使用定制的气溶胶方法合成的。该方法能够快速、高效地合成纳米载体。© 2023。作者。

In recent years, several nanocarrier synthesis methods have been developed. In cancer therapy, the use of smart nanocarriers is of interest. Smart nanocarriers respond to their environment and can release their cargo in a controlled manner under the action of internal or external stimuli. In this work, we report on the development of an aerosol-assisted method for the synthesis of curcumin-loaded chitosan/alginate-based polymeric nanocarrier (CurNCs). A custom-fabricated multi-nebulizer system was utilized for the synthesis of CurNCs. The developed system comprises three main parts a sprayer, an electric heater tunnel, and a collector. Curcumin and chitosan solutions were sprayed using a pneumatic multinebulizer into the electric heater tunnel to form chitosan-curcumin assemblies. Then, the aerosol was guided into the collector solution containing sodium alginate and tri-poly phosphate aqueous solution for further cross-linkage. The synthesized CurNCs were characterized using TEM, DLS, and FTIR techniques. The TEM size of the nanoparticles was 8.62 ± 2.25 nm. The release experiments revealed that the nanocarrier is sensitive to the environment pH as more curcumin is released at acidic pH values (as is the case for cancerous tissues) compared to physiological pH. The curcumin content of the nanocarrier was 77.27 mg g-1 with a drug loading efficiency of 62%. The in-vitro cytotoxicity of the synthesized nanocarrier was evaluated against the MCF7 breast cancer cell line. The IC50 concentrations for CurNCs and curcumin were obtained as 14.86 and 16.45 mg mL-1, respectively. The results showed that while the empty nanocarrier shows non-significant cytotoxicity, the CurNCs impact the cell culture and cause prolonged cell deaths. Overall, pH-responsive curcumin polymeric nanocarrier was synthesized using a custom fabricated aerosol-based method. The method enabled fast and feasible synthesis of the nanocarrier with high efficiency.© 2023. The Author(s).