库拉索芦荟是一种无茎植物，长度为 60-100 厘米，叶子多汁，在各个国家的不同郊区因其治疗和愈合特性而被使用。本研究旨在研究巴巴登木叶提取物（水性和乙醇性）对酵母引起的发热和乙酸引起的大鼠扭体的影响，以评估解热生物标志物及其通过计算分析进行的植物化学筛选。对于镇痛活性模型，将60只白化大鼠（160-200kg）分为四组。在4组中，对照组由6只用生理盐水治疗的大鼠（组I）组成，标准组由6只用药物双氯芬酸治疗的大鼠（组I）组成。实验组由 48 只大鼠组成，分别用 50 mg/kg、100 mg/kg、200 mg/kg 和 400 mg/kg 剂量的巴巴登木乙醇提取物和水提取物治疗（组 III.IV）。解热活性的分组与镇痛活性的分组相同。除第二组用扑热息痛治疗外，所有组均进行与镇痛活性相同的治疗。在400 mg/kg剂量下，第III组的解热和镇痛活性均表现出显着的抑制作用。 TNF-α和IL-6在400 mg/kg剂量下表现出显着的解热活性。对于分子对接，芦荟大黄素和胆甾烷醇被用作靶向蛋白质 Tnf-α 和 IL-6 的配体分子。进行了急性口服毒性研究。即使剂量为2000mg/kg也没有死亡。进行定量和定性植物化学物质筛选以检测各种植物化学物质。因此，巴巴登花叶提取物可以以药物的形式用于治疗疼痛和发烧。

Aloe barbadensis is a stemless plant with a length of 60-100 cm with juicy leaves which is used for its remedial and healing properties in different suburbs of various countries. The present study was conducted to investigate the effect of A. barbadensis leaf extract (aqueous and ethanolic) in yeast induced pyrexia and acetic acid induced writhing in rat model to evaluate the antipyretic biomarkers and its phytochemical screening with computational analysis. For analgesic activity model 60 Albino rats (160-200 kg) were divided into four groups. Of the 4 groups, control consisted of 6 rats (Group I) treated with normal saline, standard comprised of 6 rats treated with drug diclofenac (Group I). Experimental groups consisted of 48 rats, treated with A. barbadensis ethanolic and aqueous leaf extracts at doses of 50 mg/kg, 100 mg/kg, 200 mg/kg, and 400 mg/kg (Group III. IV). For antipyretic activity group division was same as in analgesic activity. All groups were treated the same as in the analgesic activity except for the second group which was treated with paracetamol. In both antipyretic and analgesic activity at the dose of 400 mg/kg, group III showed significant inhibition. TNF-α and IL-6 showed significant antipyretic activity at a dose of 400 mg/kg. For molecular docking aloe emodin and cholestanol were used as ligand molecules to target proteins Tnf-α and IL-6. Acute oral toxicity study was performed. There was no mortality even at the dose of 2000 mg/kg. Quantitative and qualitative phytochemical screening was performed for the detection of various phytochemicals. Hence, A. barbadensis leaf extracts can be used in the form of medicine for the treatment of pain and fever.