α-山竹素 (α-MG) 和指甲花酮甲醚 (LME) 具有抗菌和抗生物膜活性。本研究的目的是开发一种含有 α-MG 和 LME 加氟化物的草药牙胶，并确定其抗菌、抗生物膜形成、抗癌、抗炎、伤口愈合和牙釉质显微硬度作用。针对链球菌的抗菌测定进行了变形菌、牙龈卟啉单胞菌和白色念珠菌的检测。使用透射电子显微镜评估微生物的超微结构形态。通过肉汤微量稀释来测试对微生物生物膜形成的影响。通过MTT测定评估细胞活力。通过测量一氧化氮产生的抑制来研究抗炎作用。通过维氏显微硬度测试测量牙釉质显微硬度。用傅里叶变换光谱仪研究了釉质的化学成分。通过扫描电子显微镜和能量色散 X 射线光谱检查牙釉质表面形态和氟化物含量。结果表明，在治疗剂量下，α-MG 和 LME 对抗菌活性和抗菌膜形成具有协同作用，且没有细胞毒性。在较高剂量下，牙胶抑制癌细胞系的增殖。用牙胶加氟化物刷牙后，牙釉质显微硬度增加。牙釉质表面检测到大量氟化物。含有α-MG和LME的牙胶协同其抗菌活性和抗菌膜形成，抑制口腔癌细胞增殖。在牙胶中加入氟化物可以增加牙釉质的显微硬度。因此，含有 α-MG 和 LME 加氟化物的草药牙胶可能有助于预防龋齿和促进口腔健康。© 2023。作者。

α-Mangostin (α-MG) and lawsone methyl ether (LME) show antimicrobial and anti-biofilm activities. The objectives of this study were to develop a herbal tooth gel containing α-MG and LME plus fluoride and determine its antimicrobial, anti-biofilm formation, anti-cancer, anti-inflammatory, wound healing, and enamel microhardness effects.Antimicrobial assays against Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans were performed. The microbes' ultrastructural morphology was assessed using Transmission Electron Microscopy. The effect on microbial biofilm formation was tested by a broth microdilution. Cell viability was assessed with MTT assay. The anti-inflammatory effect was investigated by measuring inhibition of nitric oxide production. Enamel microhardness was measured via Vickers microhardness testing. The enamel chemical composition was investigated with Fourier Transform Spectrometer. The enamel surface morphology and fluoride content were examined by Scanning Electron Microscopy and Energy Dispersive X-ray Spectroscopy.The results show synergistic effects of α-MG and LME on antimicrobial activity and antibiofilm formation without cytotoxicity at a therapeutic dose. At a higher dose, the tooth gel inhibited proliferation of cancer cell line. Enamel microhardness was increased after brushing with the tooth gel plus fluoride. A large amount of fluoride was detected on the enamel surface.The tooth gel containing α-MG and LME synergized its antimicrobial activity and antibiofilm formation and inhibited oral cancer cell proliferation. Incorporating fluoride into the tooth gel increased enamel microhardness. Thus, the herbal tooth gel containing α-MG and LME plus fluoride may be useful for preventing dental caries and promoting oral health.© 2023. The Author(s).