Reactome 知识库 (https://reactome.org) 是 Elixir 和 GCBR 核心生物数据资源，提供了各种正常和疾病相关生物过程的手动策划的分子详细信息。过程被注释为单个一致数据模型中分子转换的有序网络。因此，Reactome 既可以作为手动管理的人类生物过程的数字档案，也可以作为发现数据中功能关系的工具，例如肿瘤细胞的基因表达谱或体细胞突变目录。在这里，我们回顾了整个人类蛋白质组的注释、通路背景下蛋白质和小分子药物的致病遗传变异的靶向注释以及支持细胞和组织特异性通路的明确注释的进展。最后，我们简要讨论了使 Reactome 与其他相关资源（例如基因本体论）更全面地互操作以及维护由此产生的社区资源网络所涉及的问题。© 作者 2023。由牛津大学出版社代表 Nucleic Acids Research 出版。

The Reactome Knowledgebase (https://reactome.org), an Elixir and GCBR core biological data resource, provides manually curated molecular details of a broad range of normal and disease-related biological processes. Processes are annotated as an ordered network of molecular transformations in a single consistent data model. Reactome thus functions both as a digital archive of manually curated human biological processes and as a tool for discovering functional relationships in data such as gene expression profiles or somatic mutation catalogs from tumor cells. Here we review progress towards annotation of the entire human proteome, targeted annotation of disease-causing genetic variants of proteins and of small-molecule drugs in a pathway context, and towards supporting explicit annotation of cell- and tissue-specific pathways. Finally, we briefly discuss issues involved in making Reactome more fully interoperable with other related resources such as the Gene Ontology and maintaining the resulting community resource network.© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.