PIWI相互作用RNA（piRNA）是肿瘤发生过程中出现的一种非编码RNA，在调节肿瘤微环境中发挥着关键作用。 piRNA 在调节免疫通路中的作用的系统分析对于肿瘤免疫治疗非常重要。在这项研究中，对 piRNA 进行了深入分析，以开发一种集成计算算法，即免疫学 piRNA (ImmPI) 管道，用于揭示 piRNA 的全局表达景观并确定其在免疫途径中的调节作用。免疫学piRNA显示出在免疫细胞中过度表达模式的趋势，引起肿瘤的扰动，与免疫细胞的浸润显着相关，并且具有预后价值。 ImmPI 评分有助于优先考虑肿瘤相关的 piRNA，并区分 SKCM 的两种亚型（免疫冷表型和热表型），其特点是不同的预后、免疫原性和抗肿瘤免疫。最后，我们为生物医学研究社区开发了一个交互式网络资源（ImmPI 门户：http://www.hbpding.com/ImmPi），其中包含几个有用的模块来浏览、可视化和下载免疫学 piRNA 分析的结果。总体而言，我们的工作提供了跨多种癌症类型的 piRNA 的全面概况，并阐明了它们在肿瘤免疫中的调节和功能作用。这些发现为未来基于 piRNA 的癌症免疫疗法的研究和开发铺平了道路。© 2023 作者。

PIWI-interacting RNAs (piRNAs), an emergent type of non-coding RNAs during oncogenesis, play critical roles in regulating tumor microenvironment. Systematic analysis of piRNAs' roles in modulating immune pathways is important for tumor immunotherapy. In this study, in-depth analysis of piRNAs was performed to develop an integrated computational algorithm, the immunology piRNA (ImmPI) pipeline, for uncovering the global expression landscape of piRNAs and identifying their regulatory roles in immune pathways. The immunology piRNAs show a tendency towards overexpression patterns in immune cells, causing perturbations in tumors, being significantly associated with infiltration of immune cells, and having prognostic value. The ImmPI score can contribute to prioritizing tumor-related piRNAs and distinguish two subtypes of SKCM (immune-cold and hot phenotypes), as characterized by different prognoses, immunogenicity and antitumor immunity. Finally, we developed an interactive web resource (ImmPI portal: http://www.hbpding.com/ImmPi) for the biomedical research community, with several useful modules to browse, visualize, and download the results of immunology piRNAs analysis. Overall, our work provides a comprehensive landscape of piRNAs across multiple cancer types and sheds light on their regulatory and functional roles in tumor immunity. These findings pave the way for future research and development of piRNA-based immunotherapies for cancer treatment.© 2023 The Authors.