急性髓系白血病 (AML) 是一种侵袭性血液恶性肿瘤，会导致骨髓中髓系细胞过度生成。 piwi相互作用RNA（piRNA）属于小非编码RNA，其异常表达在不同癌细胞的进展中发挥着重要作用。 hsapiR-32877 在 AML 中上调。反义 LNA GapmeR 下调 hsa-piR-32877 可能会抑制骨髓细胞增殖并诱导骨髓细胞凋亡。我们通过反义 LNA GapmeR 阻断了人骨髓母细胞和 M-07e 细胞系中 hsa-piR-32877 的表达。在 24、48 和 72 小时时用反义 LNA GapmeR 转染样品。进行定量逆转录酶聚合酶链反应 (qRT-PCR) 以研究 hsa-piR-32877、CASP3 和 CASP9 的表达。 CASP3 和 CASP9 在细胞凋亡中发挥重要作用。通过 CFSE（羧基荧光素二乙酸琥珀酰亚胺酯）测定法研究细胞增殖。结果显示，患者和细胞系样本中的 hsa-piR-32877 被反义 LNA GapmeR 下调。此外，转染后，细胞增殖和凋亡分别减少和增加。我们的数据表明 hsa-piR-32877 抑制可能作为一种抑制 AML 中人类白血病细胞增殖的新治疗方法。© 作者。

Acute myeloid leukemia (AML) is an invasive form of hematologic malignancies which results in the overproduction of myeloid cells in the bone marrow. Aberrant expression of piwi-interacting RNAs (piRNAs) which belong to small non-coding RNAs, play important roles in different cancer cells' progress. hsa- piR- 32877 is up-regulated in AML. Down regulation of hsa-piR-32877 by antisense LNA GapmeRs could be potential for suppression of myeloid cell proliferation and induce myeloid cell apoptosis. We have blocked the expression of hsa-piR-32877 by antisense LNA GapmeRs in human bone marrow blast cells, and the M-07e cell line. Samples were transfected with antisense LNA GapmeRs at 24, 48, and 72 hours. The Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to investigate the expression of hsa-piR-32877, CASP3, and CASP9. Both CASP3 and CASP9 play important roles in apoptosis. Cell proliferation was studied via CFSE (carboxyfluorescein diacetate succinimidyl ester) assay. Results showed that hsa-piR-32877 was down-regulated by antisense LNA GapmeRs in the patient and cell line samples. Also, after transfection, cell proliferation and apoptosis decreased and increased, respectively. Our data suggested that hsa-piR-32877 suppression may act as a novel therapeutic method for the inhibition of human leukemic cells proliferation in AML.© The Author(s).