乳腺癌具有起病隐匿、进展快、易复发、转移的特点。传统的单一疗法通常由于药物输送不足而无效。因此，多模式治疗与肿瘤微环境（TME）响应性纳米平台的结合越来越多地被考虑用于乳腺癌的靶向治疗。我们合成了用于协同化疗和光热疗法的生物活性混合纳米颗粒。简而言之，合成了平均粒径为 113.58 ± 2.14 nm 的阿霉素 (DOX) 和吲哚菁绿 (ICG) 纳米颗粒 (DI)，其表面用聚多巴胺 (PDA) 修饰，并附着在厌氧益生菌婴儿双歧杆菌 (Bif) 上。生物活性Bif@DIP杂化物表现出良好的光热转换效率，约为38.04%。此外，Bif 的自驱动能力允许将 PDA 涂层的 DI 纳米颗粒 (DIP) 靶向递送至肿瘤的缺氧区域。 TME中的低pH值和高GSH水平刺激Bif@DIP混合物中DOX和ICG的受控释放，然后触发肿瘤细胞凋亡并诱导免疫原性细胞死亡（ICD），从而产生有效且持续的抗肿瘤作用具有最小的全身毒性。因此，自驱动的 Bif@DIP 混合体是一种很有前景的纳米药物，可用于针对实体癌的靶向化疗和光热疗法。版权所有 © 2023 作者。由 Elsevier Masson SAS 出版。保留所有权利。

Breast cancer is characterized by insidious onset, rapid progression, easy recurrence, and metastasis. Conventional monotherapies are usually ineffective due to insufficient drug delivery. Therefore, the combination of multimodal therapy with tumor microenvironment (TME)-responsive nanoplatforms is increasingly being considered for the targeted treatment of breast cancer. We synthesized bioactive hybrid nanoparticles for synergistic chemotherapy and photothermal therapy. Briefly, doxorubicin (DOX) and indocyanine green (ICG)-loaded nanoparticles (DI) of average particle size 113.58 ± 2.14 nm were synthesized, and their surface were modified with polydopamine (PDA) and attached to the anaerobic probiotic Bifidobacterium infantis (Bif). The bioactive Bif@DIP hybrid showed good photothermal conversion efficiency of about 38.04%. In addition, the self-driving ability of Bif allowed targeted delivery of the PDA-coated DI nanoparticles (DIP) to the hypoxic regions of the tumor. The low pH and high GSH levels in the TME stimulated the controlled release of DOX and ICG from the Bif@DIP hybrid, which then triggered apoptosis of tumor cells and induced immunogenic cell death (ICD), resulting in effective and sustained anti-tumor effect with minimum systemic toxicity. Thus, the self-driven Bif@DIP hybrid is a promising nanodrug for the targeted chemotherapy and photothermal therapy against solid cancers.Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.