婴儿血管瘤（IH）是女性婴儿常见的血管肿瘤，可导致美观问题和面部疤痕。本研究旨在探讨755 nm长脉冲翠绿宝石激光对IH的抑制作用及其潜在机制。将血管瘤内皮细胞（HemECs）暴露于755 nm长脉冲翠绿宝石激光下，评估其对细胞增殖和凋亡的影响。建立患者来源的异种移植模型，以评估激光治疗对体内 IH 的抑制作用。在体外，755 nm 长脉冲紫翠玉激光有效抑制 HemEC 的增殖并诱导细胞凋亡。激光治疗显着抑制了肿瘤的体积和重量，同时血管内皮生长因子 A (VEGFA)、血管内皮生长因子受体 2 (VEGFR2)、磷脂酰肌醇 3-激酶 (PI3K) 和蛋白激酶 B (Akt) 显着下调血管瘤细胞和肿瘤中的表达水平。此外，激光治疗导致 VEGFA165a 转化为 VEGFA165b。 TUNEL 染色显示激光治疗后肿瘤细胞凋亡增加，同时 cleaved caspase-3 和 Bax 上调，Bcl-2 下调。除了选择性光热分解的原理外，VEGF/PI3K/Akt 轴的调节可能作为使用长脉冲宽度 755 nm 激光进行 IH 治疗的潜在机制。这为了解 IH 发病机制和潜在治疗靶点的分子机制提供了宝贵的线索，同时为使用激光疗法安全有效地治疗增殖性 IH 提供了理论基础。版权所有 © 2023 作者。由 Elsevier B.V. 出版。保留所有权利。

Infantile hemangioma (IH) is a common vascular tumor in female infants, which can lead to aesthetic issues and facial scarring. This study aimed to investigate the inhibitory effects and underlying mechanisms of 755 nm long-pulsed alexandrite laser on IH.Hemangioma endothelial cells (HemECs) were exposed to 755 nm long-pulsed alexandrite laser to evaluate its impact on cell proliferation and apoptosis. A patient-derived xenograft model was established to assess the inhibitory effects of laser treatment on IH in vivo.In vitro, 755 nm long-pulsed alexandrite laser effectively suppressed the proliferation of HemECs and induced cell apoptosis. Laser treatment significantly inhibited the volume and weight of tumors, accompanied by significant downregulation of vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) expression levels in both hemangioma cells and tumors. Additionally, laser treatment resulted in the conversion of VEGFA165a to VEGFA165b. TUNEL staining demonstrated increased apoptosis in tumor cells after laser treatment, along with upregulation of cleaved caspase-3 and Bax, and downregulation of Bcl-2.In addition to the principle of selective photothermal decomposition, modulation of the VEGF/PI3K/Akt axis may serve as a potential mechanism for IH treatment using a long pulse-width 755 nm laser. This sheds valuable light on the molecular mechanisms underlying IH pathogenesis and potential therapeutic targets while providing a theoretical basis for the safe and efficient management of proliferative IH using laser therapy.Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.