Clerodendrum infortunatum L. 常见于热带和亚热带国家，广泛应用于包括阿育吠陀、尤纳尼和顺势疗法在内的本土医学系统中。该植物在治疗各种炎症性疾病方面具有重要用途，包括哮喘、咳嗽、胸部不适、疼痛、风湿病、骨关节炎、胃溃疡、癌症、糖尿病、皮肤病等。研究目的：关于 C 的广泛治疗特性为了探索该植物的抗炎活性并分析其生物活性成分，我们以民间医学中的C. infortunatum为原料，采用正己烷、二氯甲烷、乙酸乙酯、甲醇、乙醇和水从C. infortunatum叶中制备连续提取物。使用膜稳定和蛋白质抗变性测定评估这些提取物的体外抗炎活性。使用丰年虾致死试验测试提取物的毒性。已进行 GC-MS 分析来分析和表征植物中存在的各种生物活性成分。每种测试的提取物均显示出显着的体外抗炎活性（p<0.05）。连续的乙酸乙酯提取物表现出最强的膜稳定和蛋白质抗变性活性，在热和低渗诱导的膜稳定和蛋白质抗变性测定中，IC50值分别为331.3、308.3和279.2μg/mL。连续甲醇提取物在热和低渗诱导的膜稳定和蛋白质抗变性测定中也表现出很强的活性，IC50 值分别为 371.5、331.6 和 284.6μg/mL。这些提取物在丰年虾致死率测定中表现出一系列毒性，正己烷、二氯甲烷、乙酸乙酯、甲醇、乙醇和水提取物的 LC50 值分别为 51.4、59.7、47.3、37.1、68.1 和 77.3μg/mL。对直接正己烷、乙酸乙酯和甲醇提取物进行 GC-MS 分析，检测到 32 种不同的化合物。主要化合物（>5%）为苯酚、3,5-双（1,1-二甲基乙基）-十六烷酸甲酯、草蒿脑、硬脂酸甲酯、羊毛甾醇、9-甲氧基双环[6.1.0]nona-2,4 ,6-三烯、苯丙酸、3,5-双(1,1-二甲基乙基)4羟甲酯、22,23-二溴豆甾醇乙酸酯、3-甲基-4-(苯硫基)-2-丙-2-烯基- 2,5-二氢噻吩1,1-二氧化物、苯甲醇、α-甲基-α-丙基-和9-十八碳烯酸(Z)-甲酯。我们的研究结果表明，C. infortunatum 富含多种生物活性植物成分和科学证据支持该植物的抗炎潜力。版权所有 © 2023。由 Elsevier B.V. 出版

Clerodendrum infortunatum L. is commonly distributed in tropical and subtropical countries and is widely used in the indigenous systems of medicine including Ayurveda, Unani, and Homeopathy. The plant has important uses in the treatment of various inflammatory disorders which includes asthma, cough, chest complaints, pain, rheumatism, osteoarthritis, gastric ulcer, cancer, diabetes, skin diseases etc. AIM OF THE STUDY: Regarding the extensive healing properties of C. infortunatum in folk medicine, we aimed to explore the anti-inflammatory activity of the plant and analyze its bioactive components.Sequential extracts were prepared from C. infortunatum leaves using n-hexane, dichloromethane, ethyl acetate, methanol, ethanol and water. In vitro anti-inflammatory activity of these extracts were evaluated using membrane stabilization and protein anti-denaturation assays. Toxicity of the extracts was tested using brine shrimp lethality assay. GC-MS analysis has been carried out to analyze and characterize various bioactive constituents present in the plant.Each of the tested extracts showed significant (p < 0.05) in vitro anti-inflammatory activity. Sequential ethyl acetate extract demonstrated the strongest membrane stabilization and protein anti-denaturation activities with IC50 values of 331.3, 308.3 and 279.2 μg/mL for heat and hypotonicity induced membrane stabilization and protein anti-denaturation assays, respectively. Sequential methanol extract also exhibited strong activity in heat and hypotonicity induced membrane stabilization and protein anti-denaturation assays with IC50 values of 371.5, 331.6 and 284.6 μg/mL, respectively. The extracts demonstrated a range of toxicity in the brine shrimp lethality assay with LC50 values of 51.4, 59.7, 47.3, 37.1, 68.1 and 77.3 μg/mL for n-hexane, dichloromethane, ethyl acetate, methanol, ethanol and water extracts respectively. The GC-MS analysis of direct n-hexane, ethyl acetate and methanol extracts detected 32 different compounds. The major compounds (>5%) were phenol, 3,5-bis(1,1-dimethylethyl)-, hexadecanoic acid methyl ester, estragole, methyl stearate, lanosterol, 9-methoxybicyclo[6.1.0]nona-2,4,6-triene, benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl) 4 hydroxy methyl ester, 22,23-dibromostigmasterol acetate, 3-methyl-4-(phenylthio)-2-prop-2-enyl-2,5-dihydrothiophene 1,1-dioxide, benzenemethanol, .alpha.-methyl-.alpha.-propyl- and 9-octadecenoic acid (Z)- methyl ester.The findings of our study revealed that C. infortunatum is rich with diverse bioactive phytoconstituents and the scientific evidences support the anti-inflammatory potential of the plant.Copyright © 2023. Published by Elsevier B.V.