全球第三大最常诊断的疾病和癌症相关死亡的第二大常见原因是结直肠癌 (CRC)。已鉴定许多人类恶性肿瘤具有 ADORA2A 高表达。然而，其在CRC中的作用仍然不明确。使用稳定转染的 SETDB1 敲低细胞的 RNA-seq 来识别差异表达的基因。此外，用siRNA在CRC细胞系SW620和HCT116中进行ADORA2A的敲低，并用质粒在SW480细胞中进行ADORA2A的过表达。采用CCK8、集落形成、伤口愈合和Transwell实验检测ADORA2A敲低和过表达后细胞增殖、迁移和侵袭的影响。此外，通过流式细胞术分析细胞凋亡，使用蛋白质印迹法检测细胞凋亡相关蛋白和关键 PI3K/AKT 通路蛋白。 ADORA2A 在 RNA-seq 分析后被鉴定，在 CRC 预后中发挥重要作用。与 SW480 细胞系相比，ADORA2A 在 SW620 和 HCT116 细胞系中相对较高。 SW620和HCT116中ADORA2A敲除抑制细胞增殖、迁移和侵袭，而ADORA2A过表达则具有相反的作用。此外，ADORA2A还影响凋亡相关蛋白的表达，包括Bcl-2、Bax、Cleaved caspase-3和Cleaved caspase-9，并减少细胞凋亡。此外，该过程可能包括PI3K/AKT信号通路。 ADORA2A 通过 PI3K/AKT 信号通路促进 CRC 进展并抑制细胞凋亡。它可能有助于 CRC 的管理和治疗。© 2023。作者。

The third most often diagnosed disease globally and the second most prevalent cause of cancer-related death is colorectal cancer (CRC). Numerous human malignancies have been identified to have high expression of ADORA2A. However, it is still ambiguous about its function in CRC. RNA-seq with stable transfected SETDB1 knockdown cells was used to identify differentially expressed genes. Further, knockdown of ADORA2A in CRC cell lines SW620 and HCT116 was performed with siRNA and over expression of ADORA2A in SW480 cells was conducted with plasmids. CCK8, colony formation, wound healing, and transwell assay were used to detect the effects of cell proliferation, migration, and invasion after knockdown and over expression of ADORA2A. Also, apoptosis was analyzed by flow cytometry, apoptosis-related proteins and key PI3K/AKT pathway proteins were detected using Western blotting. ADORA2A was identified after RNA-seq analysis and played an important role in CRC prognosis. ADORA2A was relatively high in SW620 and HCT116 cell lines compared to SW480 cell lines. ADORA2A knockdown in SW620 and HCT116 inhibited cell proliferation, migration, and invasion, while ADORA2A overexpression had the opposite effect. In addition, ADORA2A also impacted the expression of apoptosis-related proteins, including Bcl-2, Bax, Cleaved caspase-3 and Cleaved caspase-9, and reduced apoptosis. Furthermore, this process may include the PI3K/AKT signaling pathway. ADORA2A promotes CRC progression and inhibits apoptosis by the PI3K/AKT signaling pathway. It may contribute to the management and treatment of CRC.© 2023. The Author(s).