荧光侧流免疫分析 (LFA) 是乳腺癌即时检测 (POCT) 系统中的一种工具，因其快速、简单和方便而引起了人们的关注。然而，样品和组成材料在可见光区域表现出自发荧光，这是荧光LFA发展的一个非常大的障碍。生物样品的自发荧光在第二近红外 (NIR-II) 范围内几乎找不到，并且样品散射和吸收的 NIR-II 光比可见光少。在这里，我们报告了一个 NIR-II QD-LFA 平台，该平台使用 NIR-II 荧光 Ag2Se 量子点 (QD)，其发射波长为 1020 nm，封装在聚苯乙烯珠中作为荧光探针。 NIR-II LFA平台的建立可在15分钟内检测乳腺癌肿瘤标志物（CEA和CA153），检测限低（CEA：0.768 ng mL-1，CA153：1.192 U mL-1），回收率高（93.7） % ~ 108.8%），相对标准偏差（RSD）小于10%。这项研究证明了 NIR-II Ag2Se 聚苯乙烯珠作为 LFA 中荧光探针的潜力，可用于快速、准确地识别生物标志物。它们适合在专业场合使用。© 2023。作者获得 Springer-Verlag GmbH Austria（Springer Nature 旗下公司）的独家许可。

Fluorescent lateral flow immunoassay (LFA), one tool in point of care testing (POCT) systems for breast cancer, has attracted attention because it is quick, simple, and convenient. However, samples and the constituent material exhibit autofluorescence in the visible region, which is a very large obstacle in the development of fluorescent LFAs. The autofluorescence of biological samples is scarcely found in the second near-infrared (NIR-II) range and samples scatter and absorb less NIR-II light than visible light. Here, we report an NIR-II QD-LFA platform using the NIR-II fluorescent Ag2Se quantum dots (QDs) with 1020 nm emission encapsulated into polystyrene beads as fluorescent probes. The NIR-II LFA platform was established to detect breast cancer tumour markers (CEA and CA153) within 15 min with a low limit of detection (CEA: 0.768 ng mL-1, CA153: 1.192 U mL-1), high recoveries (93.7% ~ 108.8%), and relative standard deviations (RSDs) of less than 10%. This study demonstrated the potential of NIR-II Ag2Se polystyrene beads as a fluorescent probe in LFA for rapid and accurate identification of biomarkers. They are suited for use in professional situations.© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.