Stratifin (SFN) 的异常表达与许多肿瘤的发生和进展密切相关。本研究旨在探讨SFN是否通过LIMK2/Cofilin信号通路调控宫颈癌细胞的转移。本研究比较了SFN在正常宫颈组织和宫颈癌组织中的表达情况。我们建立了 SFN 过表达和 SFN 沉默细胞模型，以使用 Transwell 和划痕实验评估宫颈癌细胞的侵袭和迁移能力。 YO-PRO-1/PI 和 EdU 染色用于评估细胞凋亡和增殖能力，而 Actin-Tracker Green-488 用于研究细胞骨架重塑。通过蛋白质印迹和免疫荧光检查SFN、LIMK2、p-LIMK2、Cofilin和p-Cofilin的表达水平。我们的研究结果表明，宫颈鳞状细胞癌组织中 SFN 的表达升高。 SFN 过表达可增强宫颈癌细胞的侵袭和迁移、诱导细胞骨架重塑、促进细胞增殖并抑制细胞凋亡。此外，SFN 过表达上调了 LIMK2、p-LIMK2、Cofilin 和 p-Cofilin 的表达水平。相反，沉默 SFN 会产生相反的效果。 SFN在宫颈癌的诊断中发挥着重要作用。 SFN 可以通过 LIMK2/Cofilin 信号传导调节宫颈癌细胞增殖、凋亡、细胞骨架重塑和转移。© 2023。作者。

The aberrant expression of Stratifin (SFN) is intricately associated with the initiation and progression of numerous tumors. This study aims to investigate whether SFN regulates the metastasis of cervical cancer cells through the LIMK2/Cofilin signaling pathway. In this study, we compared the expression of SFN in normal cervical tissues and cervical carcinoma tissues. We established SFN overexpression and SFN silencing cellular models to assess the invasive and migratory capabilities of cervical cancer cells using transwell and scratch assays. YO-PRO-1/PI and EdU staining were employed to evaluate apoptotic and proliferative capacities, while Actin-Tracker Green-488 was utilized to investigate cytoskeletal remodeling. The expression levels of SFN, LIMK2, p-LIMK2, Cofilin, and p-Cofilin were examined through Western blotting and immunofluorescence. Our findings revealed elevated expression of SFN in cervical squamous cell carcinoma tissues. SFN overexpression was observed to enhance invasion and migration of cervical cancer cells, induce cytoskeletal remodeling, facilitate cell proliferation, and suppress apoptosis. Furthermore, SFN overexpression upregulated the expression levels of LIMK2, p-LIMK2, Cofilin, and p-Cofilin. Conversely, silencing SFN exerted opposite effects. SFN plays an important role in the diagnosis of cervical cancer. SFN can regulate cervical cancer cell proliferation, apoptosis, cytoskeletal remodeling and metastasis through LIMK2/Cofilin signaling.© 2023. The Author(s).