岩藻糖基转移酶（FUT）分子已被确定参与恶性肿瘤的致癌过程。然而，岩藻糖基转移酶-3 (FUT3) 在肺腺癌 (LUAD) 恶性表型中的生物学功能仍不清楚。在此，我们研究了FUT3与LUAD病理过程之间的关联。通过免疫化学、RT-qPCR和蛋白质印迹法来评估FUT3在LUAD和相应癌旁组织中的表达。 FUT3 的预后价值通过 Kaplan-Meier 绘图仪数据库进行评估。通过GSEA研究了FUT3在LUAD中的生物学过程和潜在机制。此外，还进行了免疫荧光和代谢物活性检测，以确定 FUT3 在 LUAD 葡萄糖代谢中的潜在作用。通过蛋白质印迹法检测与 NF-κB 信号通路相关的活性生物标志物。通过皮下肿瘤模型分析FUT3对LUAD肿瘤发生的影响。与个体的邻近组织相比，FUT3在LUAD组织中显着上调。 FUT3 过表达可能预示 LUAD 患者预后不良。 FUT3 的敲低显着抑制了 LUAD 细胞中的肿瘤增殖、迁移和糖代谢改变。此外，GSEA 证明 FUT3 升高与 NF-κB 信号通路呈正相关。此外，体外和体内试验还表明，FUT3 的下调可通过 NF-κB 通路失活来抑制肿瘤发生和葡萄糖代谢。我们的研究结果表明，FUT3 通过 NF-κB 信号传导参与 LUAD 的葡萄糖代谢过程和肿瘤发生途径。 FUT3 可能是诊断和治疗 LUAD 患者的最佳靶标。© 2023。作者。

Fucosyltransferases (FUTs) molecules have been identified to be involved in carcinogenesis of malignant tumors. Nevertheless, the biological function of fucosyltransferases-3 (FUT3) in lung adenocarcinoma (LUAD) malignant phenotype remains unclear. Herein, we investigated the association between FUT3 and LUAD pathological process.Immunochemistry, RT-qPCR and western blot assays were conducted to evaluate the expression of FUT3 in LUAD and corresponding adjacent tissues. The prognostic value of FUT3 was assessed via Kaplan‑Meier plotter database. The biological process and potential mechanism of FUT3 in LUAD were conducted via GSEA. Additionally, immunofluorescence and metabolite activity detection were performed to determine the potential role of FUT3 in LUAD glucose metabolism. The active biomarkers associated with NF-κB signaling pathway were detected via western blot. Subcutaneous tumor model was conducted to analyze the effect of FUT3 on tumorigenesis of LUAD.FUT3 was remarkably upregulated in LUAD tissues compared with adjacent tissues from individuals. FUT3 overexpression may predict poor prognosis of LUAD patients. Knockdown of FUT3 significantly inhibited tumor proliferation, migration and glucometabolic alteration in LUAD cells. Moreover, GSEA demonstrated that elevated FUT3 was positively related to NF-κB signaling pathway. Additionally, in vitro and in vivo assays also indicated that downregulation of FUT3 resulted in the suppression of oncogenesis and glucose metabolism via inactivation of NF-κB pathway.Our findings demonstrated that FUT3 was involved in glucometabolic process and tumorigenesis of LUAD via NF-κB signaling pathway. FUT3 may be an optimal target for diagnosis and treatment of LUAD patients.© 2023. The Author(s).