树脂灵芝和类似蛇的真菌化学特性和生物活性仍未被探索。本研究评估了灵芝和蛇舌草提取物的抗氧化、细胞毒性和细胞迁移能力，然后进行了植物化学分析。进行 MTT 测定以确定细胞毒性以及人类癌细胞系中的细胞迁移研究。通过 DPPH 和 FRAP 测定评估抗氧化特性。此外，还进行了 LC-MS/MS 分析，以阐明负责抗癌和抗氧化活性的植物化学物质。在 1% 和 5% 浓度的树脂砂提取物中，针对 HCT 116 细胞系观察到显着的浓度依赖性细胞毒性，分别为 12.7% 和 13.7%。同样，在 1% 和 5% 浓度的 S. similis 提取物中，分别观察到显着的浓度依赖性细胞毒性，分别为 6.7% 和 25.5%。 G.resinaceum 和 S.similis 的提取物均显示出更好的抗肺癌细胞迁移潜力。两种提取物均表现出良好的 DPPH 和三价铁离子自由基清除活性。 LC-MS 分析揭示了 11 种来自 S. similis 的化合物和 15 种来自 G. resinaceum 子实体的化合物。萜类化合物、生物碱、细胞毒性肽和其他代谢物等化合物被确定为两种提取物的主要成分。这些提取物表现出针对 HCT 116 癌细胞的细胞毒活性，以及​​中等的抗氧化活性。这意味着提取物可用作制药和食品工业中的生物活性天然来源。

Mycochemical properties and bioactivities of Ganoderma resinaceum and Serpula similis remain unexplored. The present study assessed antioxidant, cytotoxicity, and cell migration abilities of Ganoderma and Serpula extracts, followed by their phytochemical analyses. The MTT assay was conducted to determine the cytotoxicity along with the cell migration studies in human cancer cell lines. The antioxidant profiles were evaluated through DPPH and FRAP assays. Furthermore, LC-MS/MS analysis was performed to elucidate the phytochemicals responsible for anticancer and antioxidant activities. Significant concentration-dependent cytotoxicities of 12.7% and 13.7% were observed against HCT 116 cell lines at 1% and 5% concentrations of the G. resinaceum extract, respectively. Similarly, significant concentration-dependent cytotoxicities of 6.7% and 25.5% were observed at 1% and 5% concentrations of the S. similis extract, respectively. The extracts of G. resinaceum and S. similis both shows better anti-migration potential in lung cancer cells. Both extracts demonstrated good scavenging activity on DPPH and ferric ion free radicals. LC-MS analysis revealed 11 compounds from S. similis and 15 compounds from G. resinaceum fruiting bodies. Compounds such as terpenoids, alkaloids, cytotoxic peptides, and other metabolites were identified as major components in both extracts. These extracts exhibited cytotoxic activity against HCT 116 cancer cells, along with moderate antioxidant activity. This implies that the extracts might be used as bioactive natural sources in the pharmaceutical and food industries.