在无效输血的情况下，目前使用 Luminex 平台进行 HLA 抗体检测和表征的固相免疫分析来选择 HLA 相容性血小板产品。一种新的 HLA 抗体鉴定方法，即 HISTO SPOT® HLA AB 测试（BAG Health care GmbH，利希，德国），基于通过 HISTO MATCH HLA AB 模块检测到的彩色斑点来检测针对重组单抗原 (SA) 的抗体软件在 MR.SPOT® 上全自动运行。本研究的目的是比较 HISTO SPOT HLA AB 和 C1qScreen™ (C1q SAB) 检测与 Labscreen 单抗原 I 类 (OL SAB) 检测检测 56 份血清样本中抗 HLA I 类抗体的能力来自 54 名血小板难治性急性髓系白血病患者，他们在单一肿瘤血液学中心接受了 HLA 不匹配血小板浓缩液。 OL SAB 测试总共检测到 1414 个 I 类特异性，其中 433 个 HLA-A 和 981 个 HLA-B。 874 种抗原的平均荧光强度 (MFI) > 5000，655 种抗原的平均荧光强度 (MFI) >< 5000。 HISTO SPOT® HLA AB 和 C1q SAB 测试分别识别了 85% 和 79% 的 MFI >5000 的 OL SA 检测到的抗原，但未识别 ​​34% 和 44% 的 OL SAB 检测到的抗原，凸显了较低的灵敏度这些技术。有趣的是，通过 HISTO SPOT® HLA AB 和 C1q SAB 检测鉴定的供体特异性抗体 (DSA) 与 HLA 不匹配血小板浓缩物发生反应，具有与 OL SAB 检测相同的特异性 (86%) 和阳性预测值 (77%)当 DSA 检测的 MFI 阈值 >2000 时。尽管 HISTO SPOT® HLA AB 测试的灵敏度不如 OL SAB 测试，但该测试可用于选择 HLA 兼容的血小板产品。© 2023 作者。 HLA：免疫反应遗传学，约翰·威利 (John Wiley) 出版

In an ineffective transfusion context, solid-phase immunoassays using the Luminex platform for the detection and characterization of HLA antibodies are currently used to select HLA-compatible platelet products. A new HLA antibody identification method, the HISTO SPOT® HLA AB test (BAG Health care GmbH, Lich, Germany), based on the detection of antibodies directed against a recombinant single antigen (SA) by colored spots detected by HISTO MATCH HLA AB module software, runs fully automated on the MR.SPOT®. The aim of this study was to compare the ability of the HISTO SPOT HLA AB and C1qScreen™ (C1q SAB) assays with that of the Labscreen single antigen class I (OL SAB) assay to detect anti-HLA class I antibodies in 56 serum samples from 54 platelet refractory acute myeloid leukemia patients who received HLA mismatch platelet concentrates at a single oncohematology center. In total, 1414 class I specificities, 433 HLA-A and 981 HLA-B, were detected by the OL SAB test. The mean fluorescence intensity (MFI) was >5000 for 874 antigens and <5000 for 655 antigens. The HISTO SPOT® HLA AB and C1q SAB tests identified 85% and 79% of OL SA-detected antigens with an MFI >5000, respectively, but did not identify 34% and 44% of OL SAB-detected antigens, highlighting the lower sensitivity of these techniques. Interestingly, the donor-specific antibodies (DSAs) identified by the HISTO SPOT® HLA AB and C1q SAB assays reacted against HLA mismatch platelet concentrates with the same specificity (86%) and positive predictive (77%) value as in the OL SAB test when the MFI threshold was >2000 for DSA detection. Although the HISTO SPOT® HLA AB test is less sensitive than the OL SAB test, this test could be used for the selection of HLA-compatible platelet products.© 2023 The Authors. HLA: Immune Response Genetics published by John Wiley & Sons Ltd.