由于澳大利亚羽扇豆品种富含多酚、膳食纤维、优质蛋白质和丰富的生物活性化合物，具有显着的抗氧化、抗糖尿病和抗癌活性，本研究工作旨在研究九个羽扇豆品种的结肠癌缓解活性通过抗增殖实验、细胞凋亡测定和细胞凋亡机制鉴定对HCT116和HT29结肠癌细胞系的影响。预先筛选了九个品种的 HCT116 和 HT29 细胞抗增殖能力，同时考虑了热处理对癌细胞活力的影响。 Mandelup 和 Jurien 对 HCT116 细胞显示出显着的抑制作用，而 Jurien 和 Mandelup 对 HT29 细胞增殖的抑制作用最高。加工大大降低了抗增殖活性。在Annexin V-FITC测定中，羽扇豆品种Mandelup、Barlock和Jurien（剂量：300 μg/mL）诱导结肠癌细胞的早期和晚期凋亡。探讨了细胞凋亡的机制，其中涉及 HCT116 细胞（Mandelup 和 Barlock）和 HT29 细胞（Jurien 和 Mandelup）中 caspase-3/7 激活和细胞内活性氧 (ROS) 生成的增强。因此，研究结果表明，羽扇豆品种通过诱导结直肠癌细胞凋亡来阻止细胞周期，而结直肠癌细胞是由ROS生成增加和caspases-3/7激活引发的。

As Australian lupin cultivars are rich sources of polyphenols, dietary fibers, high-quality proteins, and abundant bioactive compounds with significant antioxidant, antidiabetic, and anticancer activities, this research work is aimed at investigating the colon cancer alleviation activity of nine cultivars of lupin seeds on HCT116 and HT29 colon carcinoma cell lines through anti-proliferation assay, measurement of apoptosis, and identification of the mechanism of apoptosis. Nine cultivars were pre-screened for anti-proliferation of HCT116 and HT29 cells along with consideration of the impact of heat processing on cancer cell viability. Mandelup and Jurien showed significant inhibition of HCT116 cells, whereas the highest inhibition of HT29 cell proliferation was attained by Jurien and Mandelup. Processing decreased the anti-proliferation activity drastically. Lupin cultivars Mandelup, Barlock, and Jurien (dose: 300 μg/mL) induced early and late apoptosis of colon cancer cells in Annexin V-FITC assay. The mechanism of apoptosis was explored, which involves boosting of caspases-3/7 activation and intracellular reactive oxygen species (ROS) generation in HCT116 cells (Mandelup and Barlock) and HT29 cells (Jurien and Mandelup). Thus, the findings showed that lupin cultivars arrest cell cycles by inducing apoptosis of colorectal carcinoma cells triggered by elevated ROS generation and caspases-3/7 activation.