老年人群中骨相关疾病的发病率较高，极大地影响患者的生活质量。在整个研究中，我们合成了 CeO2 生物复合纳米材料。通过 XRD、TEM 和 SEM 研究了 CeO2 纳米粒子（CeO2 NPs）的独特结构特征。检测骨肉瘤细胞系 (MG-63) 纳米颗粒的 ALP 酶水平、成骨细胞中的关键蛋白，并用茜素红 S 染色，以评估骨肉瘤细胞系的物理特性、生物活性和钙沉积。此外，我们使用H2O2构建氧化应激模型来评估CeO2纳米粒子的抗氧化活性。实验数据表明，CeO2 NPs 增加了 MG-63 细胞的抗氧化能力，并显着增加了碱性磷酸酶活性、钙沉积和骨生长，表现为骨分化蛋白 BMP2、OCN、OPN 和 I 型胶原表达增加。有趣的是，RNA干扰和功能恢复实验证实，CeO2 NPs增强了与NRF2信号传导相关的MG-63细胞的抗氧化活性。总之，该材料有望成为骨相关疾病的潜在治疗方法。© 2023。作者获得 Springer Science Business Media, LLC（Springer Nature 旗下公司）的独家许可。

The incidence of bone-related diseases is higher in the elderly population, which greatly affects the patients' quality of life. Throughout this research, we synthesized a biocomposite nanomaterial of CeO2. The unique structural characteristics of CeO2 nanoparticles (CeO2 NPs) were studied by means of XRD, TEM, and SEM. Nanoparticles of an osteosarcoma cell line (MG-63) were assayed for ALP enzyme levels, key proteins in osteoblasts, and stained with Alizarin Red S to assess the physical properties, bioactivity, and calcium deposition of the osteosarcoma cell line. Moreover, we used H2O2 to construct an oxidative stress model to evaluate the antioxidant activity of CeO2 NPs. Experimental data showed that the CeO2 NPs increased the antioxidant capacity of MG-63 cells and significantly increased alkaline phosphatase activity, calcium deposition, and bone growth as manifested by increased expression of bone differentiation proteins BMP2, OCN, OPN, and type I collagen. Interestingly, RNA interference and functional recovery experiments confirmed that CeO2 NPs enhanced the antioxidant activity of MG-63 cells related to NRF2 signaling. In conclusion, the material is expected to be a potential treatment for bone-related diseases.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.