基底细胞癌（BCC）是最常见的皮肤癌类型。由于多种潜在的潜在分子肿瘤畸变，临床治疗方案尚不明确。本研究基于 RNA 和蛋白质组分析展示了人类 BCC 的多位点分子异质性概况。对从 9 名患者身上切除的三个病变区域进行了分析。重点是基于同一患者肿瘤内异质性的蛋白质组和 RNA 测量以及不同患者结节性、浸润性和浅表性 BCC 肿瘤亚型的肿瘤间异质性的基因表达谱。我们观察到蛋白质组和 RNA 表达谱的肿瘤内和肿瘤间变异性显着重叠，显示 BCC 肿瘤中蛋白质表达的显着多位点异质性。亚型间分析还确定了每种 BCC 亚型的独特蛋白质。这种分析可以使人们更深入地了解 BCC 分子异质性，并可能有助于开发新的采样工具，用于 BCC 的个性化诊断治疗方法。版权所有：© 2023 Berl 等人。这是一篇根据知识共享署名许可条款分发的开放获取文章，允许在任何媒体上不受限制地使用、分发和复制，前提是注明原始作者和来源。

Basal cell carcinoma (BCC) is the most common type of skin cancer. Due to multiple, potential underlying molecular tumor aberrations, clinical treatment protocols are not well-defined. This study presents multisite molecular heterogeneity profiles of human BCC based on RNA and proteome profiling. Three areas from lesions excised from 9 patients were analyzed. The focus was gene expression profiles based on proteome and RNA measurements of intra-tumor heterogeneity from the same patient and inter-tumor heterogeneity in nodular, infiltrative, and superficial BCC tumor subtypes from different patients. We observed significant overlap in intra- and inter-tumor variability of proteome and RNA expression profiles, showing significant multisite heterogeneity of protein expression in the BCC tumors. Inter-subtype analysis has also identified unique proteins for each BCC subtype. This profiling leads to a deeper understanding of BCC molecular heterogeneity and potentially contributes to developing new sampling tools for personalized diagnostics therapeutic approaches to BCC.Copyright: © 2023 Berl et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.