黄芪救逆汤（HQJND）是安徽医科大学第一附属医院创制的中西医结合治疗严重烧伤的方剂。它由12种草药组成，在临床上已经使用了几十年。大大缩短了病程，但黄芪健脾汤治疗急性肾病的具体机制仍不清楚。因此，本研究的目的是利用现代药理学工具论证黄芪健脾汤治疗急性肾病的疗效和机制。在这项研究中，首先使用液相色谱串联质谱法（LC-MS/MS）检测了HQJND中的化学成分。然后，利用网络药理学筛选药物和疾病作用的靶点，预测药物治疗疾病过程中作用的信号通路。最后，我们通过建立动物模型、中药灌胃治疗、采集大鼠肾脏和血清检测肾功能、实时定量聚合酶链反应（RT-qPCR）来验证该药物的疗效并探讨其治疗机制。 、蛋白质印迹（WB）、苏木精和伊红（HE）染色和免疫组织化学（IHC）。采用液相色谱串联质谱分析红芪健脾汤中的14种重要活性成分，同时进行网络药理学筛选，鉴定出353个疾病相关标记基因以及286个药物靶点，最终确定了TNF/NF-κB（肿瘤坏死因子/核因子κ-B）信号位点：红芪健脾干预烧伤急性肾损伤的关键通路。大鼠血清肾功能和组织病理学结果表明，使用HQJND可显着改善严重烧伤的肾功能。 RT-qPCR和WB证实模型组大鼠TNF/NF-κB信号通路被激活，而HQJND可以抑制该信号通路，因为它调节了该过程中关键蛋白的表达。一种有效的草药制剂，可改善严重烧伤后的肾功能损伤，肾功能最有可能通过 TNF/NF-κB 信号通路发挥作用。版权所有 © 2023。由 Elsevier B.V. 出版。

Huangqi Jiuni decoction (HQJND) is a prescription for the treatment of severe burns provided based on traditional Chinese and Western medicine, which is created by the First Affiliated Hospital of Anhui Medical University. It consists of 12 herbs and has been used clinically for decades. It has greatly shortened the course of the disease, but the mechanism by which HQJND treats the disease still remains unclear.Hence, the objective of this investigation was to utilize modern pharmacological tools to demonstrate the efficacy and mechanism of HQJND in the treatment of acute kidney injury (AKI) caused by severe burns.In this study, the chemical constituents in HQJND were first examined using liquid chromatography tandem mass spectrometry (LC-MS/MS). Then, by using network pharmacology, we screened the targets of drug and disease action, and predicted the signaling pathways acting in the course of drug treatment of disease. Finally, we attempted to verify the efficacy of the drug and explored its therapeutic mechanism after the establishment of an animal model, herbal gavage treatment, collection of rat kidneys and serum for renal function, quantitative real-time Polymerase Chain Reaction (RT-qPCR), Western Blotting (WB), Hematoxylin and eosin (HE) staining and Immunohistochemistry (IHC).The 14 important active ingredients in HQJND was analyzed by liquid chromatography tandem mass spectrometry, while network pharmacology screening was performed to identify 353 disease-associated marker genes and 286 drug targets, finally identifying the TNF/NF-κB (tumor necrosis factor/nuclear factor kappa-B) signaling site: the key pathway of burn-induced acute kidney injury when HQJND intervened. The serum renal function and histopathology of rats demonstrated that the use of HQJND significantly improved the renal function in severe burns. RT-qPCR and WB confirmed that the TNF/NF-κB signaling pathway was activated in the Model group of rats, and HQJND could curb the signaling pathway because it moderated the expressions of key proteins in the process.Based on modern pharmacology, we explored an effective herbal preparation to ameliorate the impairment of renal function after severe burns, which is most likely to function through the TNF/NF-κB signaling pathway.Copyright © 2023. Published by Elsevier B.V.