月季丙酮提取物对耐药菌株和肺癌细胞系的抗菌和抗癌功效。
Anti-microbial and anti-cancer efficacy of acetone extract of Rosa chinensis against resistant strain and lung cancer cell line.
发表日期：2023 Nov 10
作者：
Kalaivani Madhavaram Kuppusamy, Sivakumar Selvaraj, Pujithaa Singaravelu, Cordelia Mano John, Kalaiselvan Racheal, Keziaann Varghese, Dinesh Kaliyamoorthy, Elumalai Perumal, Krishnamoorthy Gunasekaran
来源：
Cell Death & Disease
摘要：
筛选草药植物的各种治疗特性是时候了，因为它显示出有希望的活性。该植物药理活性的科学证据加强了植物的传统应用。采购玫瑰花（Rosa chinensis）并将其磨成粗粉。从玫瑰花中分离DNA，并通过rbcL-BF和rbcL-724R引物进行分子鉴定。使用纸片和琼脂扩散方法评估抗菌活性，并使用 MTT 测定在肺癌细胞系中检查玫瑰花提取物 (RE) 的抗癌作用。使用吖啶橙/溴化乙锭染色和赫斯特染色定性测量 RE 诱导的细胞死亡机制。使用 GC-MS-5975C 进行 GC-MS 分析。RE 对各种 ATCC 培养物显示出有效的抗菌活性。玫瑰提取物强烈抑制 ESBL 抗性生物体的生长，同时抑制 ESBL 抗性生物体中生物膜的形成。该提取物导致肺癌细胞凋亡和坏死。 GC-MS 分析表明存在多种生物活性化合物，如克林霉素、植醇、辛酸和豆甾醇，这可能是该植物具有治疗特性的原因。这项研究显示了对临床分离菌株的抗菌和生物膜抑制活性。肺炎克雷伯菌。该研究显示了 A549 癌细胞系的细胞毒性和凋亡活性。因此，该植物可以作为对抗耐药菌株的有效抗菌药物。© 2023。作者。
Screening of herbal plants for various therapeutic properties is the hour as it shows promising activity. Scientific evidence of the pharmacological activity of the plant strengthens the traditional application of plants.Rose flowers (Rosa chinensis) were procured and grounded into a coarse powder. The DNA was isolated from rose flower and molecular identification was performed by rbcL-BF and rbcL-724R primers. Antibacterial activity was evaluated by using disc and agar diffusion methods and the anti-cancer effect of the rose flower extract (RE) was examined using MTT assay in lung cancer cell line. The mechanism of cell death induced by RE was qualitatively measured using Acridine orange/Ethidium bromide staining and Hoechst staining. GC-MS analysis was performed using GC-MS-5975C.The RE showed potent antimicrobial activity against various ATCC cultures. The rose extract strongly inhibits the growth of ESBL resistant organism along with inhibition of biofilm formation in the ESBL resistant organism. The extract caused apoptotic and necrotic cell death in lung cancer cells. GC-MS analysis demonstrated the presence of several biologically active compounds such as Clindamycin, Phytol, Octanoic acid, and Stigmasterol which might be the reason for the therapeutic properties of the plant.This study shows the antimicrobial and biofilm inhibition activity against the clinical isolates of Klebsiella pneumonia. The study shows the cytotoxic and apoptotic activity in A549 cancer cell line. Thus, the plant may act as a potent antimicrobial drug against resistant strains.© 2023. The Author(s).