本研究旨在探讨贯叶连翘橄榄油提取物对人结直肠癌细胞和人骨髓间充质干细胞的细胞毒性和转移特性的影响。此外，还通过 qRT-PCR 和蛋白质印迹分析评估了 ALDH3A1 和 Vimentin 的表达。使用基于分光光度法的方法测量了通过油富集制备的甲醇提取物的总酚和类黄酮含量以及抗氧化活性。通过MTT测定评估提取物对SW-480和骨髓间充质干细胞的细胞毒作用，结果IC50值分别为4.8mg/ml和4.9mg/ml。已确定，在针对 SW-480 和人骨髓来源的间充质干细胞测定的 IC50 值下，细胞迁移和集落形成显着减少。

This study is aimed to investigate the effects of Hypericum perforatum olive oil extract on the cytotoxic and metastatic properties of human colorectal cancer cells and human bone marrow-derived mesenchymal stem cells. In addition, ALDH3A1 and Vimentin expressions were evaluated by qRT-PCR and western blot analysis. Total phenolic and flavonoid contents and antioxidant activity of methanol extracts prepared with oil enrichment were measured using spectrophotometry-based methods. The cytotoxic effects of the extracts on SW-480 and bone marrow-derived mesenchymal stem cells were evaluated by MTT assay, resulting in IC50 values of 4.8 mg/ml and 4.9 mg/ml, respectively. It was determined that cell migration and colony formation were significantly reduced at the IC50 values determined for SW-480 and human bone marrow-derived mesenchymal stem cells.