小鼠和人类胰腺导管腺癌组织切片外植体的生成和离体培养。
Generation and ex vivo culture of murine and human pancreatic ductal adenocarcinoma tissue slice explants.
发表日期：2023 Nov 10
作者：
Amanda R Decker-Farrell, Alice Ma, Fangda Li, Alexander Muir, Kenneth P Olive
来源：
Cellular & Molecular Immunology
摘要：
传统的 2D/3D 共培养模型通常不能反映胰腺导管腺癌 (PDAC) 肿瘤的细胞异质性，而体内模型可能速度缓慢且不适合机制研究。在这里，我们提出了使用组织切片外植体培养小鼠 PDAC 外植体和相应的人类 PDAC 模型的协议。我们描述了海绵生产、介质和材料的制备、组织收集和切片的步骤。然后，我们详细介绍了外植体铺板、日常培养和样品收集的程序。版权所有 © 2023 作者。由爱思唯尔公司出版。保留所有权利。
Traditional 2D/3D co-culture models typically do not reflect the cellular heterogeneity of pancreatic ductal adenocarcinoma (PDAC) tumors, while in vivo models can be slow and ill-suited to mechanistic investigations. Here, we present a protocol for culturing murine PDAC explants and a corresponding human PDAC model using tissue slice explants. We describe steps for sponge production, preparation of media and materials, tissue collection, and sectioning. We then detail procedures for explant plating, daily culture, and collection of samples.Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.