移植模型提供了通过使用相应的转基因供体动物来评估感兴趣的分子与肿瘤细胞外渗的相关性的机会。在这里，我们提出了一种小鼠原位单肺移植方案，作为肺转移研究的工具。我们描述了动物准备、肺移植和肿瘤细胞注射的步骤。然后，我们详细介绍了直接比较转基因左肺和幼稚右肺实质之间肿瘤细胞扩散的程序。有关该协议的使用和执行的完整详细信息，请参阅 Giannou 等人。 (2023).1.版权所有 © 2023 作者。由爱思唯尔公司出版。保留所有权利。

The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.