慢性肺部疾病是一个主要且日益严重的全球健康问题，通常由香烟烟雾引起。我们的目的是探讨乳酸菌 (LAB) 对支气管上皮细胞中香烟烟雾的抗氧化作用。在香烟烟雾刺激的 BEAS-2B 细胞和 3-D 支气管球中测试了 21 种热灭活 (HK) LAB 菌株的抗氧化作用类器官。我们发现 HK 植物乳杆菌 BGPKM22 具有抗香烟烟雾的抗氧化活性、对过氧化氢的抵抗力以及自由基中和活性。我们证明 HK BGPKM22 抑制香烟烟雾诱导的芳基碳氢化合物受体 (AhR) 和核因子红细胞 2 相关因子 2 (Nrf2) 基因的表达。 BGPKM22的无细胞上清液（SN）充分证实了HK BGPKM22的作用。我们首次揭示了植物乳杆菌BGPKM22的HK和SN具有抗氧化活性，并调节暴露于香烟烟雾。© 作者 2023。由牛津大学出版社代表应用微生物学国际出版。

Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells.The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke stimulated BEAS-2B cells and 3-D bronchospheres organoids. We showed that HK Lactiplantibacillus plantarum BGPKM22 possesses antioxidant activity against cigarette smoke, resistance to hydrogen peroxide, and free radical neutralizing activity. We demonstrated that HK BGPKM22 inhibited cigarette smoke induced expression of the Aryl hydrocarbon receptor (AhR) and Nuclear factor erythroid 2 related factor 2 (Nrf2) genes. The cell-free supernatant (SN) of BGPKM22 fully confirmed the effects of HK BGPKM22.For the first time, we revealed that HK and SN of L. plantarum BGPKM22 possess antioxidant activity and modulate AhR and Nrf2 gene expression in bronchial epithelial cells exposed to cigarette smoke.© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.