抗生素使用不当导致全球耐药生物膜细菌增多。因此，研究人员对具有高度生物相容性和低毒性的绿色材料越来越感兴趣。在此，通过在己烷包水乳液工艺下交联源自猕猴桃的 DNA 的伯胺和芳香醛（异丙苯醛、对茴香醛或香草醛），合成了具有亚胺键的纳米凝胶 (NG)。透射电子显微镜显示NGs具有球形几何形状，平均粒径范围为40至140nm，zeta电位显示负电荷。此外，在细胞活力测试中，DNA-芳香醛 NG 对正常细胞类器官和人红细胞表现出较低的细胞毒性。这些 NG 还针对四种致病菌进行了各种检测测试。从最低抑菌浓度测定中可以看出，DNA-香草醛 (DNA-VA) NG 在极低的抑制浓度下对细菌表现出显着的抗菌作用。扫描电镜观察发现细菌发生变形，免疫印迹检测细胞内groEL蛋白表达。与这些结果一致，DNA-芳香醛NGs成功地保护了秀丽隐杆线虫免受铜绿假单胞菌诱导的致死。这些 DNA NG 为抗菌芳香醛的束缚提供了多价 3D 空间，增强了它们与细菌壁的相互作用。这些结果为未来新型抗生素的开发提供了新方向。版权所有 © 2023。由 Elsevier B.V. 出版。

Improper use of antibiotics has led to the global rise of drug-resistant biofilm bacteria. Thus, researchers have been increasingly interested in green materials that are highly biocompatible and have low toxicity. Here, nanogels (NGs) with imine bonds were synthesized by crosslinking kiwifruit-derived DNA's primary amine and aromatic aldehydes (cuminaldehyde, p-anisaldehyde, or vanillin) under water-in-hexane emulsion processes. Transmission electron microscope showed that the NGs had spherical geometry with an average particle size ranging from 40 to 140 nm and that the zeta potential indicated a negative charge. Additionally, the DNA-aromatic aldehyde NGs showed low cytotoxicity toward normal cell organoids and human RBCs in cell viability tests. These NGs were also tested against four pathogenic bacteria for various assays. DNA-vanillin (DNA-VA) NGs exhibited significant antibacterial effects against bacteria with very low inhibitory concentrations as seen in a minimum inhibitory concentration assay. Scanning electron microscope observation revealed that the bacteria were deformed, and immunoblotting detected intracellular groEL protein expression. In agreement with these results, DNA-aromatic aldehyde NGs successfully protected C. elegans from P. aeruginosa-induced lethality. These DNA NGs provided a multivalent 3D space for antibacterial aromatic aldehydes to tether, enhancing their interaction with the bacterial wall. These results offer a new direction for the development of novel antibiotics in the future.Copyright © 2023. Published by Elsevier B.V.