茶和茶制品作为世界上最受欢迎的饮料被广泛使用。 EGCG是绿茶中含量最丰富的生物活性茶多酚，对预防和治疗糖尿病具有积极作用。然而，EGCG 暴露对成年小鼠葡萄糖稳态和胰岛的影响尚未有报道。在这项研究中，我们研究了成年雄性小鼠通过饮用水暴露于 1 和 10 mg/kg/天 EGCG 60 天后的葡萄糖稳态以及胰岛 α 和 β 细胞的形态和分子变化。两个 EGCG 组的血糖稳态均未受到影响。 10 mg/kg/day EGCG组β细胞中胰腺十二指肠homebox1（Pdx1）的表达上调，这可能与胰岛素水平、β细胞质量和β细胞增殖增加有关。 α细胞中aristaless相关同源框（Arx）的表达没有显着变化，这与α细胞质量不变相对应。两个 EGCG 组中肌肉腱膜纤维肉瘤癌基因同源物 B (MafB) 阳性 α 细胞的显着减少可能与胰高血糖素水平降低有关。这些结果表明，补充 EGCG 会剂量依赖性地增加成年小鼠的 β 细胞质量，并影响血清胰岛素和胰高血糖素的水平。我们的研究结果表明，健康人经常喝茶可能有预防糖尿病的可能性。版权所有 © 2023。由 Elsevier Inc. 出版。

Tea and tea products are widely used as the most popular beverage in the world. EGCG is the most abundant bioactive tea polyphenol in green tea, which has positive effects on the prevention and treatment of diabetes. However, the impact of EGCG exposure on glucose homeostasis and islets in adult mice have not been reported. In this study, we studied glucose homeostasis and the morphological and molecular changes of pancreatic islet α and β cells in adult male mice after 60 days of exposure to 1 and 10 mg/kg/day EGCG by drinking water. Glucose homeostasis wasn't affected in both EGCG groups. The expression of pancreatic duodenal homebox1 (Pdx1) in β cells was upregulated, which might be related to increased insulin level, β cell mass and β cell proliferation in 10 mg/kg/day EGCG group. The expression of aristaless-related homeobox (Arx) in α cells didn't change significantly, which corresponded with the unchanged α-cell mass. The significant reduction of musculoaponeurotic fibrosarcoma oncogene homolog B (MafB) positive α-cells might be associated with decreased glucagon level in both EGCG groups. These results suggest that EGCG supplementation dose-dependent increases β cell mass of adult mice and affects the levels of serum insulin and glucagon. Our results show that regular tea drinking in healthy people may have the possibility of preventing diabetes.Copyright © 2023. Published by Elsevier Inc.