1 型牛α疱疹病毒 (BoAHV-1) 与呼吸系统和生殖系统综合症有关。迄今为止，BoAHV-1 流产涉及的免疫机制尚不为人所知。我们研究了实验接种 BoAHV-1 的牛的胎盘和胎儿肺中先天免疫反应的关键要素。通过组织病理学分析这些组织。此外，通过qPCR进行病毒鉴定，并通过免疫组织化学评估肿瘤坏死因子-α、白细胞介素1-α等炎症细胞因子和诱导型一氧化氮合酶和环氧甘酶-2等炎症介质的表达。通过qPCR在胎盘和胎儿器官中检测到BoAHV-1，显示轻度炎症病变，证实病毒经胎盘感染。受感染胎儿和胎盘的肺部、肺实质中的肿瘤坏死因子-α 和胎儿附件中的环氧甘酶 2 的诱导型一氧化氮合酶免疫标记水平较高。然而，白细胞介素1-α在这些器官中的表达较弱。据我们所知，这是第一项提供有力证据证明胎儿对 BoAHV-1 感染的早期免疫反应的研究。需要进一步了解 BoAHV-1 感染期间胎儿-母体单位复杂的免疫相互作用的知识，以澄清流产的发病机制。版权所有 © 2023 Elsevier B.V. 保留所有权利。

Bovine alphaherpesvirus type 1 (BoAHV-1) is associated with respiratory and reproductive syndromes. Until present the immunologic mechanisms involved in BoAHV-1 abortion are partially known. We studied key elements of the innate immune response in the placentas and fetal lungs from cattle experimentally-inoculated with BoAHV-1. These tissues were analyzed by histopathology. Furthermore, virus identification was performed by qPCR and the expression of the inflammatory cytokines such as tumor necrosis factor-alpha, interleukin 1-alpha and inflammatory mediators like inducible nitric oxide synthase and cyclooxeganse-2 was evaluated by immunohistochemistry. The viral transplacental infection was confirmed by the detection of BoAHV-1 by qPCR in the placenta and fetal organs, which revealed mild inflammatory lesions. Inducible nitric oxide synthase immunolabelling was high in the lungs of infected fetuses and placentas, as well as for tumor necrosis factor-alpha in the pulmonary parenchyma and cyclooxeganse-2 in fetal annexes. However, the expression of interleukin 1-alpha was weak in these organs. To our knowledge, this is the first study that provides strong evidence of an early immune response to BoAHV-1 infection in the conceptus. Advances in the knowledge of the complex immunological interactions at the feto-maternal unit during BoAHV-1 infection are needed to clarify the pathogenesis of abortion.Copyright © 2023 Elsevier B.V. All rights reserved.