葡萄糖转运蛋白1（GLUT-1）的表达水平与肿瘤恶性程度高度相关，使其成为癌症治疗的一个有前景的治疗靶点。 GLUT-1表达水平的检测对于癌症发现和评估药物治疗的疗效具有重要意义。然而，目前的 GLUT-1 检测方法主要依赖于传统技术。因此，开发非破坏性体内监测系统对于评估 GLUT-1 表达和肿瘤对各种药物的反应具有不可估量的价值。本研究利用还原氧化石墨烯-多壁碳纳米管复合材料（rGO-MWCNT）作为导电涂层，以甲苯胺蓝O（TBO）-石墨烯-金纳米粒子为基础，建立了检测活细胞上GLUT-1的电化学平台。 -GLUT-1抗体作为电化学探针。该传感器在检测细胞上的 GLUT-1 方面表现出优异的性能，线性范围为 10 - 105 个细胞/mL，具有良好的稳定性和选择性。该传感器成功检测了多种肿瘤细胞类型中的GLUT-1表达，包括经过siRNA或药物处理的细胞，结果与流式细胞术、蛋白质印迹和免疫荧光等传统方法获得的结果一致。该传感器在评估肿瘤细胞的恶性程度、区分肿瘤细胞中的葡萄糖摄取途径、降低医疗成本以及促进电化学传感技术向临床应用的转化方面具有广阔的前景。版权所有 © 2023 Elsevier B.V. 保留所有权利。

The expression level of glucose transporter 1 (GLUT-1) is highly correlated with tumor malignancy, making it a promising therapeutic target for cancer treatment. The detection of GLUT-1 expression level is significant for cancer discovery and valuating the efficacy of drug treatments. However, current methods for GLUT-1 detection primarily rely on traditional techniques. Therefore, the development of anon-destructive in vivo monitoring system would be invaluable for assessing GLUT-1 expression and tumor responses to various drugs. In this study, an electrochemical platform for detection of GLUT-1 on living cells was established using reduced graphene oxide-multi-wall carbon nanotube composite (rGO-MWCNT) as a conductive coating and toluidine blue O (TBO)-graphene-gold nanoparticle-GLUT-1 antibody as the electrochemical probe. The sensor demonstrated excellent performance in detecting GLUT-1 on cells with a linear range of 10 - 105 cells/mL, good stability and selectivity. The sensor successfully detected GLUT-1 expressions in multiple tumor cell types, including those treated with siRNA or drugs, and the results were consistent with those obtained from traditional methods such as flow cytometry, western blotting, and immunofluorescence. The sensor is promising in evaluating the malignant level of tumor cells, distinguishing glucose uptake pathways in tumor cells, reducing medical costs, and facilitating the translation of electrochemical sensing technology to the clinical settings.Copyright © 2023 Elsevier B.V. All rights reserved.