该研究旨在确定基于 CpG 甲基化的结直肠癌 (CRC) 预后的预后生物标志物和预测模型，并在大型外部队列中对其进行验证。我们进行了系统性搜索，分析了 28 项研究中的 298 个独特的 CpG 和 12 个基于 CpG 的预后模型。调整临床变量后，48 个 CpG 和 5 个预后模型被证实与生存相关。然而，模型的辨别能力不足，受试者工作特征曲线下面积在0.53至0.62之间。校准准确性大多较差，并且没有观察到超出传统临床变量的显着附加预后价值。所有预后模型也被评为具有高偏倚风险。虽然一小部分 CpG 显示出潜在的临床实用性和普遍性，但基于 CpG 的预后模型表现不佳且缺乏临床相关性。版权所有 © 2023。由 Elsevier B.V. 出版。

The research aimed to identify CpG-methylation-based prognostic biomarkers and prediction models for colorectal cancer (CRC) prognosis and validate them in a large external cohort. A systematic search was conducted, analyzing 298 unique CpGs and 12 CpG-based prognostic models from 28 studies. After adjustment for clinical variables, 48 CpGs and five prognostic models were confirmed to be associated with survival. However, the discrimination ability of the models was insufficient, with area under the receiver operating characteristic curves ranging from 0.53 to 0.62. Calibration accuracy was mostly poor, and no significant added prognostic value beyond traditional clinical variables was observed. All prognostic models were also rated at high risk of bias. While a fraction of CpGs showed potential clinical utility and generalizability, the CpG-based prognostic models performed poorly and lacked clinical relevance.Copyright © 2023. Published by Elsevier B.V.