配体N-{[(苯基氨基)硫代甲基]肼基羰基甲基}三甲基氯化铵(PTHAC)由异硫氰酸苯酯和Girard-T(三甲基铵乙酰肼)以摩尔比(1:1)回流制备。合成并纯化了NiCl2·6H2O、CuCl2·2H2O和Co(CH3COO)2·6H2O的金属配合物。使用元素分析、磁矩、光谱（IR、UV-Vis）等多种仪器性能对 PTHAC 及其 Cu(II)、Co(II) 和 Ni(II) 金属配合物 (1-3) 进行了表征、 1H NMR、质量）和热分析（TGA 和 DTG）。元素分析、磁矩、光谱（IR、UV-Vis、1H NMR、质量）和热分析（TGA 和 DTA）的结果提供了以下结构：产生的配体及其(1-3)络合物。根据光谱结果，PTHAC 作为 O、N 和 S 三齿供体，与铜 (II)、钴 (II) 和镍 (II) 离子形成具有八面体几何形状的单核配合物。所有原子性质，包括键长、键角、HOMO、LUMO、偶极矩和电荷，均已确定。 PTHAC 和产生的 (1-3) 复合物针对乳腺癌细胞的细胞毒活性已被研究并与分子模型相关。与游离配体 CoII-L 和 NiII-L 相比，CuII-L 复合物更有效地抑制乳腺癌细胞生长。此外，PTHAC 配体还成功应用于使用油酸表面活性剂 (HOL) 通过浮选和分光光度法测定多种天然水、经过认证的矿石和药物样品中 Co(II) 的分离。在 pH 6.5 时，PTHAC 与 Co(II) 反应生成深绿色 (1:1) Co(II):PTHAC 复合物，使用油酸 (HOL) 表面活性剂可显着漂浮该复合物。影响分离程序的不同实验变量，例如对 pH、Co(II) 浓度、HOL、PTHAC、温度等进行了研究。 Co(II) 的线性范围为 (0.1-7.0) mgL-1。在水层和浮渣层中，有色络合物的摩尔吸光系数分别为 0.14 × 104 和 0.16 × 105Lmol-1 cm-1。 LOD 为 0.04 mgL-1，与 3.7 × 10-3 µg cm-2 的 Sandell 灵敏度相关，预富集因子为 200，RSD% (n = 5) 小于 4.2%。此外，还研究了PTHAC与Cu(II)、Co(II)和Ni(II)的配位过程以及使用HOL表面活性剂浮选PTHAC-Co(II)络合物过程中涉及的机理。阐明。© 2023。作者。

The ligand N-{[(phenyl amino) thioxomethyl] hydrazino carbonyl methyl} trimethyl ammonium chloride (PTHAC) was prepared by the refluxing phenyl isothiocyanate and Girard-T (trimethyl ammonium-acethydrazide) in a molar ratio (1:1). The metal complexes derived from NiCl2.6H2O, CuCl2.2H2O and Co(CH3COO)2.6H2O were synthesized and purified. The PTHAC and its Cu(II), Co(II), and Ni(II) metal complexes(1-3) were characterized using a variety of various instrumental performances such as elemental analysis, magnetic moment, spectra (IR, UV-Vis, 1H NMR, mass) and thermal analysis (TGA and DTG).The results of element analysis, magnetic moment, spectra (IR, UV-Vis, 1H NMR, mass), and thermal (TGA and DTA) analyses provide the structures of the produced ligand and its (1-3) complexes. According to the spectroscopic results, PTHAC acts as an O, N and S tridentate donor, creating a mononuclear complex with copper(II), cobalt(II), and nickel(II) ions with an octahedral geometry. All of the atomic properties, including bond lengths, bond angles, HOMO, LUMO, dipole moments, and charges, have been determined. The cytotoxic activities of the PTHAC and the produced (1-3) complexes against breast carcinoma cells have been studied and correlated to the molecular modeling. When compared to the free ligand, CoII-L, and NiII-L, the CuII-L complex inhibits breast cancer cell growth more effectively. Furthermore, the PTHAC ligand was successfully applied for separation via flotation and spectrophotometric determination of Co(II) in several natural water, certified ore and pharmaceutical samples using oleic acid surfactant (HOL). At pH 6.5, PTHAC reacted with Co(II) to create a dark green (1:1) Co(II):PTHAC complex that was floated significantly using oleic acid (HOL) surfactant. The different experimental variable affecting the separation procedure e.g. pH, concentration of Co(II), HOL, PTHAC, temperature etc.…, were investigated. Co(II) had a linear range of (0.1-7.0) mgL-1. In the aqueous and scum layers, the molar absorptivities for the coloured complex are 0.14 × 104 and 0.16 × 105Lmol-1 cm-1, respectively. The LOD was 0.04 mgL-1, which is related to Sandell sensitivity of 3.7 × 10-3 µg cm-2 with a preconcentration factor of 200 and a RSD, % (n = 5) less than 4.2%. In addition, the mechanisms involved in the process of coordination of PTHAC with Cu(II), Co(II) and Ni(II) and the mechanism involved in the process of flotation of the PTHAC-Co(II) complex using HOL surfactant were elucidated.© 2023. The Author(s).