细胞膜内蛋白质的时空组织可以影响许多生物功能，包括细胞信号传导、通讯和运输。在各种疾病中都观察到了与正常空间排列的偏差，更好地理解这一过程是推进临床干预措施发展的关键垫脚石。然而，考虑到所涉及的纳米长度尺度，检测这些微妙的变化主要依赖于复杂的超分辨率和单分子成像方法。在这项工作中，我们展示了一种替代荧光成像策略，用于检测基于材料的蛋白质组织，该材料表现出独特的光物理行为，称为聚集诱导发射（AIE）。有机AIE分子靠近时发射信号增强，分子运动受到限制。这一特性同时解决了限制传统宽场荧光成像的高背景噪声和低检测信号的问题。为了证明这种方法的潜力，荧光分子传感器与人表皮生长因子受体 2 (HER2) 特异性抗体结合，用于研究 HER2 聚集在 HER2 过表达乳腺癌细胞膜中的时空行为。值得注意的是，使用简单的宽视场荧光显微镜成功检测到 FDA 批准的单克隆抗体治疗剂（曲妥珠单抗）对 HER2 簇的破坏。虽然此处演示的传感器针对 HER2 聚类检测进行了优化，但它是一个易于调整的平台。此外，考虑到与宽场成像的兼容性，该系统有可能与高通量成像技术一起使用，加速对膜蛋白时空组织的研究。

The spatiotemporal organization of proteins within the cell membrane can affect numerous biological functions, including cell signaling, communication, and transportation. Deviations from normal spatial arrangements have been observed in various diseases, and a better understanding of this process is a key stepping stone to advancing development of clinical interventions. However, given the nanometer length scales involved, detecting these subtle changes has primarily relied on complex super-resolution and single-molecule imaging methods. In this work, we demonstrate an alternative fluorescent imaging strategy for detecting protein organization based on a material that exhibits a unique photophysical behavior known as aggregation-induced emission (AIE). Organic AIE molecules have an increase in emission signal when they are in close proximity, and the molecular motion is restricted. This property simultaneously addresses the high background noise and low detection signal that limit conventional widefield fluorescent imaging. To demonstrate the potential of this approach, the fluorescent molecule sensor is conjugated to a human epidermal growth factor receptor 2 (HER2)-specific antibody and used to investigate the spatiotemporal behavior of HER2 clustering in the membrane of HER2-overexpressing breast cancer cells. Notably, the disruption of HER2 clusters in response to an FDA-approved monoclonal antibody therapeutic (Trastuzumab) is successfully detected using a simple widefield fluorescent microscope. While the sensor demonstrated here is optimized for sensing HER2 clustering, it is an easily adaptable platform. Moreover, given the compatibility with widefield imaging, the system has the potential to be used with high-throughput imaging techniques, accelerating investigations into membrane protein spatiotemporal organization.