本工作采用超声辅助酶法提取刺梨果多糖（RPs）。在最佳提取条件下，RPs的最高提取率为4.78±0.10%。获得了名为 RP1 和 RP3 的两个纯化组分，并通过 FT-IR、单糖组成、分子量分布、甲基化和 2D NMR 光谱分析的组合策略进行系统表征。结构分析表明，RP1的主链由I型鼠李糖半乳糖醛酸(RG-I)组成，而侧链则富含阿拉伯半乳聚糖和半乳糖。 RP3 由长同型半乳糖醛酸 (HG) 主链组成，其中散布着 RG-I 结构域的交替序列，并具有半乳糖和阿拉伯糖侧链。 RP1和RP3在体外以剂量依赖性方式诱导MCF-7细胞凋亡，尤其是RP1，而对L929细胞没有影响。此外，还揭示了可能的抗癌机制，结果表明RP1通过ROS依赖性途径和线粒体途径诱导细胞凋亡。这项工作的结果不仅为RPs的应用提供了一种高效的提取方法和理论基础，而且可能有助于开发用于预防和治疗人类乳腺癌疾病的新型功能食品或药品。版权所有©2023。出版者爱思唯尔有限公司

In this work, Rosa roxburghii Tratt fruit polysaccharides (RPs) were extracted by ultrasound-assisted enzymatic method. The highest extraction yield of RPs was 4.78 ± 0.10 % under the optimal extraction conditions. Two purified fractions named RP1 and RP3 were obtained and systematically characterized by a combination strategy of FT-IR, monosaccharide composition, molecular weight distribution, methylation and 2D NMR spectroscopy analyses. Structural analysis showed that the main chain of RP1 was composed of rhamnogalacturonan type I (RG-I), while the side chains were rich in arabinogalactan and galactose. RP3 was composed of long homogalacturonan (HG) backbone interspersed with alternating sequences of RG-I domains, with galactose and arabinose side chains. RP1 and RP3 induced apoptosis of MCF-7 cells in a dose dependent manner in vitro especially for RP1, and had no effect on L929 cells. Furthermore, the possible anticancer mechanisms were revealed, and results suggested that RP1 induced apoptosis through ROS-dependent pathway and mitochondrial pathway. The results of this work not only provided an efficient extraction method and theoretical basis for the application of RPs, but also may contribute to develop novel functional foods or pharmaceutical products for the prevention and treatment of human breast cancer disease.Copyright © 2023. Published by Elsevier B.V.