非热等离子体 (NTP) 是一种由中性和带电活性物质、电场和紫外线辐射组成的电离气体。 NTP具有较低的放电温度，因为它的特点是离子和中性粒子的温度值远低于电子的温度值。反应性物质（原子、自由基、离子、电子）在 NTP 中产生并传递到生物体，在细胞或组织中诱导一系列生化过程。 NTP 可以以强度和时间依赖的方式调节活性氧 (ROS) 水平。 ROS 稳态在动物健康中发挥着重要作用。 NTP介导的ROS水平较低或生理水平促进细胞增殖和分化，而NTP介导的ROS水平较高或过高会导致氧化应激损伤甚至细胞死亡。适当条件下的NTP处理不仅可以直接产生中等水平的外源ROS并刺激细胞内ROS的生成，而且可以间接调节细胞内ROS水平，从而影响动物不同细胞和组织的氧化还原状态。然而，NTP的处理条件需要优化，并且NTP介导的ROS在不同生物靶点中的潜在机制仍不清楚。在过去的十年中，人们对 NTP 技术在生物学和医学领域的应用的兴趣迅速增长。由于其安全、无毒、高效，NTP可被开发用于伤口愈合、口腔治疗、癌症治疗和生物医学材料等广泛应用。此外，NTP与其他方法的联合应用，由于具有更有效的杀菌和抗癌能力，是目前的研究热点。有趣的是，NTP技术近年来在畜牧业领域展现出巨大的应用潜力。然而，NTP的广泛应用与不同且复杂的机制有关，NTP介导的ROS是否在其应用中发挥着关键作用尚待阐明。因此，本文主要总结了ROS对动物健康的影响，NTP介导的ROS水平通过抗氧化清除和ROS生成的机制，以及NTP介导的ROS在动物生长和繁殖、动物健康、动物源性等方面的潜在应用。食品安全和生物医学领域，包括伤口愈合、口腔治疗、癌症治疗和生物材料。这将为促进畜牧业健康发展以及动物和人类疾病的防治提供理论依据。

Non-thermal plasma (NTP) is an ionized gas composed of neutral and charged reactive species, electric fields, and ultraviolet radiation. NTP presents a relatively low discharge temperature because it is characterized by the fact that the temperature values of ions and neutral particles are much lower than that of electrons. Reactive species (atoms, radicals, ions, electrons) are produced in NTP and delivered to biological objects induce a set of biochemical processes in cells or tissues. NTP can mediate reactive oxygen species (ROS) levels in an intensity- and time-dependent manner. ROS homeostasis plays an important role in animal health. Relatively low or physiological levels of ROS mediated by NTP promote cell proliferation and differentiation, while high or excessive levels of ROS mediated by NTP cause oxidative stress damage and even cell death. NTP treatment under appropriate conditions not only produces moderate levels of exogenous ROS directly and stimulates intracellular ROS generation, but also can regulate intracellular ROS levels indirectly, which affect the redox state in different cells and tissues of animals. However, the treatment condition of NTP need to be optimized and the potential mechanism of NTP-mediated ROS in different biological targets is still unclear. Over the past ten decades, interest in the application of NTP technology in biology and medical sciences has been rapidly growing. There is significant optimism that NTP can be developed for a wide range of applications such as wound healing, oral treatment, cancer therapy, and biomedical materials because of its safety, non-toxicity, and high efficiency. Moreover, the combined application of NTP with other methods is currently a hot research topic because of more effective effects on sterilization and anti-cancer abilities. Interestingly, NTP technology has presented great application potential in the animal husbandry field in recent years. However, the wide applications of NTP are related to different and complicated mechanisms, and whether NTP-mediated ROS play a critical role in its application need to be clarified. Therefore, this review mainly summarizes the effects of ROS on animal health, the mechanisms of NTP-mediated ROS levels through antioxidant clearance and ROS generation, and the potential applications of NTP-mediated ROS in animal growth and breeding, animal health, animal-derived food safety, and biomedical fields including would healing, oral treatment, cancer therapy, and biomaterials. This will provide a theoretical basis for promoting the healthy development of animal husbandry and the prevention and treatment of diseases in both animals and human beings.