胃癌，特别是腺癌，是一个重大的全球健康问题。环境危险因素，如幽门螺杆菌感染和饮食，在其发展中发挥着作用。本研究旨在表征马兜铃的化学成分并评估其体外抗菌和抗肿瘤活性。前布瓦斯。叶的甲醇提取物（AOME）。此外，还分析了胃癌细胞系的形态变化。使用 HPLC-MS/MS 分析 AOME，并使用肉汤微量稀释法评估其对幽门螺杆菌的抗菌活性。确定 MIC 和 MBC 值，并在评估中纳入阳性和阴性对照。使用 AGS、KATO-III 和 SNU-1 癌细胞系通过体外实验评估抗癌效果。通过 SEM 和 TEM 分析检查形态变化。 AOME 含有多种化合物，包括咖啡酸、芦丁和金丝桃苷。该提取物对幽门螺杆菌表现出显着的抗菌作用，其 MIC 和 MBC 值一致为 3.70 ± 0.09 mg/mL。 AOME 以剂量和时间依赖性方式降低所有胃癌细胞的细胞活力。形态学分析显示所有肿瘤细胞系均发生显着的超微结构变化，表明细胞凋亡的发生。这项研究表明，AOME 对幽门螺杆菌具有抗菌活性，并且对胃癌细胞系具有有效的抗肿瘤特性。 AOME 有望成为胃癌治疗创新营养保健方法的自然资源。需要进一步的研究和体内研究来验证其潜在的临床应用。

Gastric cancer, particularly adenocarcinoma, is a significant global health concern. Environmental risk factors, such as Helicobacter pylori infection and diet, play a role in its development. This study aimed to characterize the chemical composition and evaluate the in vitro antibacterial and antitumor activities of an Aristolochia olivieri Colleg. ex Boiss. Leaves' methanolic extract (AOME). Additionally, morphological changes in gastric cancer cell lines were analyzed. AOME was analyzed using HPLC-MS/MS, and its antibacterial activity against H. pylori was assessed using the broth microdilution method. MIC and MBC values were determined, and positive and negative controls were included in the evaluation. Anticancer effects were assessed through in vitro experiments using AGS, KATO-III, and SNU-1 cancer cell lines. The morphological changes were examined through SEM and TEM analyses. AOME contained several compounds, including caffeic acid, rutin, and hyperoside. The extract displayed significant antimicrobial effects against H. pylori, with consistent MIC and MBC values of 3.70 ± 0.09 mg/mL. AOME reduced cell viability in all gastric cancer cells in a dose- and time-dependent manner. Morphological analyses revealed significant ultrastructural changes in all tumor cell lines, suggesting the occurrence of cellular apoptosis. This study demonstrated that AOME possesses antimicrobial activity against H. pylori and potent antineoplastic properties in gastric cancer cell lines. AOME holds promise as a natural resource for innovative nutraceutical approaches in gastric cancer management. Further research and in vivo studies are warranted to validate its potential clinical applications.