长非编码RNA（lncRNA）可以通过与微小RNA（miRNA）相互作用直接或间接调节基因表达。竞争性内源RNA使得lncRNA在肿瘤发生和进展过程中的作用更加复杂。然而，对于肺腺癌（LUAD），lncRNA 作为潜在生物标志物的预后价值及其作为竞争性内源性 RNA 的功能作用尚未得到明确描述。在本研究中，通过查询癌症基因组图谱 (TCGA) 数据库中 517 个组织样本的 LUAD 数据集，在 cBioPortal 上分析了 lncRNA 和 miRNA 的异常表达谱。总共鉴定出 92 个 lncRNA 和 125 个具有高度遗传改变的 miRNA。进一步进行生物信息学分析，构建了 LUAD 相关的 lncRNA-miRNA-mRNA ceRNA 网络，其中包括 24 个高度改变的 lncRNA、21 个 miRNA 和 142 个 mRNA。该网络中的一些关键 lncRNA 随后被鉴定为与 LUAD 预后相关，其中 CASC15 和 LINC01600 均表现出 LUAD 关于 OS 和复发的潜在预后特征。综合分析表明，LINC01600的表达与KRAS突变和淋巴结转移显着相关，CASC15和LINC01600显着倾向于共现，这可能是由于这2种lncRNA共表达的基因相似所致。我们的研究结果为更好地理解 LUAD 发病机制中的 ceRNA 调控机制提供了新的见解，并有助于识别潜在的预后生物标志物。版权所有 © 2023 作者。由 Wolters Kluwer Health, Inc. 出版

Long noncoding RNAs (lncRNAs) can directly or indirectly regulate gene expression through interacting with microRNAs (miRNAs). Competitive endogenous RNAs render the roles of lncRNAs more complicated in the process of tumor occurrence and progression. However, the prognostic value of lncRNAs as potential biomarkers and their functional roles as competitive endogenous RNAs have not been clearly described for lung adenocarcinoma (LUAD). In the present study, the aberrant expression profiles of lncRNAs and miRNAs were analyzed at cBioPortal by interrogating LUAD dataset from The Cancer Genome Atlas (TCGA) database with 517 tissue samples. A total of 92 lncRNAs and 125 miRNAs with highly genetic alterations were identified. Further bioinformatics analysis was performed to construct a LUAD-related lncRNA-miRNA-mRNA ceRNA network, which included 24 highly altered lncRNAs, 21 miRNAs and 142 mRNAs. Some key lncRNAs in this network were subsequently identified as LUAD prognosis-related, and of those, CASC15 and LINC01600 both performed the potential prognostic characteristics with LUAD regarding OS and recurrence. Comprehensive analysis indicated that the expression of LINC01600 was significantly associated with KRAS mutation and lymph node metastasis, and CASC15 and LINC01600 were significantly tended towards co-occurrence, which may be due to the similarity of genes co-expressed by these 2 lncRNAs. Our findings provided novel insight into better understanding of ceRNA regulatory mechanisms in the pathogenesis of LUAD and facilitated the identification of potential biomarkers for prognosis.Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.