LMO2 是参与 B 细胞个体发育的相关基因，也是侵袭性大 B 细胞淋巴瘤 (aLBCL) 的生存预测因子。大多数评估 LMO2 mRNA 表达的研究都依赖于微阵列平台或 qRT-PCR 方法，忽略了组织形态。在本研究中，我们通过显色原位杂交 (CISH) 评估正常组织和一系列 82 个 aLBCL 中的 LMO2 RNA 表达。LMO2 CISH 在福尔马林固定石蜡包埋的组织中进行，通过三种不同的方法评分，并与转录组面板。我们获得了将评估方法与 LMO2 蛋白表达和基因表达结果相关的统计显着结果。正常扁桃体组织显示出高水平的 LMO2，特别是在生发中心的亮区。相反，在 aLBCL 中，LMO2 表达显着减少，尤其是在携带 MYC 重排的病例中。此外，通过总生存期和 Cox 回归生存分析，结合国际预后指数数据和 LMO2 表达水平，获得了显着的结果。我们展示了一种通过 CISH 识别 LMO2 mRNA 表达的可靠方法，有效捕获了许多已报道的 LMO2 生物学特征。

LMO2 is a relevant gene involved in B-cell ontogeny and a survival predictor of aggressive large B-cell lymphomas (aLBCL). Most studies assessing LMO2 mRNA expression have relied on microarray platforms or qRT-PCR methods, overlooking tissue morphology. In this study, we evaluate LMO2 RNA expression by chromogenic in situ hybridization (CISH) in normal tissue and in a series of 82 aLBCL.LMO2 CISH was performed in formalin-fixed paraffin-embedded tissues, scored by three different methods, and correlated with a transcriptome panel.We obtained statistically significant results correlating the methods of evaluation with LMO2 protein expression and gene expression results. Normal tonsil tissue showed high levels of LMO2, particularly within the light zone of the germinal center. Conversely, in aLBCL, a notable reduction in LMO2 expression was noted, remarkably in cases carrying MYC rearrangements. Furthermore, significant results were obtained through overall survival and Cox regression survival analysis, incorporating International Prognostic Index data alongside LMO2 expression levels.We show a reliable method to identify LMO2 mRNA expression by CISH, effectively capturing many of the reported biologic features of LMO2.