缺氧可以通过影响免疫细胞的功能和活性来调节免疫系统，可能导致免疫反应改变。本研究使用各种免疫调节试剂盒，研究了白血病母细胞中白血病来源的树突状细胞 (DCleu) 的生成，以及它们在低氧 (5-10% O2) 与常氧 (21% O2) 条件下对免疫细胞激活的影响。结果表明，低氧和常氧条件下 DC/DCleu 的生成相似，生成的 DC/DCleu 的频率没有观察到显着差异。此外，研究表明，与混合淋巴细胞培养后的对照相比，当富含 T 细胞的免疫反应细胞与 Kit I 和 M 产生的 DC/DCleu 共培养时，免疫细胞的激活及其抗白血病活性得到改善。 MLCWB-DC Kit M 后，与常氧条件相比，低氧条件下的抗白血病活性得到了改善。这些发现表明，在低氧条件下使用试剂盒对白血病全血进行 DC/DCleu 培养，可产生可比的 DC/DCleu 频率，甚至可以增加与常氧条件相比的抗白血病活性。总体而言，这项研究强调了利用 DC/DCleu（可能通过试剂盒在体内诱导）作为增强急性髓系白血病患者免疫反应的有前景方法的潜力。

Hypoxia can modulate the immune system by affecting the function and activity of immune cells, potentially leading to altered immune responses. This study investigated the generation of leukemia-derived dendritic cells (DCleu) from leukemic blasts and their impact on immune cell activation under hypoxic (5-10% O2) compared to normoxic (21% O2) conditions using various immunomodulatory Kits. The results revealed that DC/DCleu-generation was similar under hypoxic and normoxic conditions, with no significant differences observed in frequencies of generated DC/DCleu. Furthermore, the study showed that the activation of immune cells and their anti-leukemic activity improved when T cell-enriched immunoreactive cells were co-cultured with DC/DCleu which were generated with Kit I and M compared to the control after mixed lymphocyte cultures. The anti-leukemic activity was improved under hypoxic compared to normoxic conditions after MLCWB-DC Kit M. These findings suggest that DC/DCleu-cultures of leukemic whole blood with Kits under hypoxic conditions yield comparable frequencies of DC/DCleu and can even increase the anti-leukemic activity compared to normoxic conditions. Overall, this research highlights the potential of utilizing DC/DCleu (potentially induced in vivo with Kits) as a promising approach to enhance immune response in patients with acute myeloid leukemia.