已在 Ophelia 综合征患者中鉴定出针对神经元抗原代谢型谷氨酸受体 5 (mGluR5) 的自身抗体，该综​​合征描述了副肿瘤性边缘脑炎和霍奇金淋巴瘤 (HL) 的同时发生。关于 HL 中 mGluR5 的频率和功能及其在引起血清阳性副肿瘤性疾病中的潜在作用的数据很少。我们使用免疫组织化学和荧光染色研究了一组具有代表性的儿科 HL 和 NHL 患者 (n = 57)，以研究 mGluR5 的表达。所有淋巴瘤组织均显示 mGluR5 染色阳性，重点是霍奇金-里德-斯滕伯格 (H-RS) 细胞。我们没有在无肿瘤淋巴结中检测到任何 mGluR5 染色，这与非恶性 B 和 T 细胞的 RNA 测序数据中不存在 GRM5 转录本一致。儿科淋巴瘤的频繁出现与其他恶性肿瘤中 mGluR5 表达和相关肿瘤进展的报道一致。我们测试了与临床特征的相关性，重点关注疾病进展和神经系统症状。 H-RS 细胞中 mGluR5 的低表达与年轻患者年龄（<15 岁）和 EBV 感染的阳性组织学相关。副肿瘤或神经系统症状仅见于 HL 患者。虽然 mGluR5 对 HL 严重程度的影响仍然可能，但 mGluR5 表达水平的预后价值需要进一步研究。

Autoantibodies targeting the neuronal antigen metabotropic glutamate receptor 5 (mGluR5) have been identified in patients with Ophelia syndrome, which describes a co-occurrence of paraneoplastic limbic encephalitis and Hodgkin lymphoma (HL). Little data exist regarding frequency and function of mGluR5 in HL and its potential role in causing seropositive paraneoplastic disease. We studied a representative cohort of pediatric HL and NHL patients (n = 57) using immunohistochemistry and fluorescence staining to investigate mGluR5 expression. All lymphoma tissues displayed positive mGluR5 staining, with focus on Hodgkin-Reed-Sternberg (H-RS) cells. We did not detect any mGluR5 staining in tumor-free lymph nodes, which is consistent with the absence of GRM5 transcripts in RNA-sequencing data from non-malignant B and T cells. The frequent presence in pediatric lymphoma falls in line with reports of mGluR5 expression and associated tumor progression in other malignancies. We tested for correlation with clinical features, focusing on disease progression and neurological symptoms. Low mGluR5 expression in H-RS cells correlated with young patient age (<15 years) and positive histology for EBV infection. Paraneoplastic or neurological symptoms were found exclusively in HL patients. While an impact of mGluR5 on HL severity remains possible, a prognostic value of mGluR5 expression levels requires further investigation.