生物学和转录组水平的异质性对低级别胶质瘤 (LGG) 的定义和分型提出了挑战，因此迫切需要特定的分子特征来增强 LGG 的诊断、治疗和预后评估。本研究重点关注脂肪酸代谢（FAM）相关基因和预后特征，探讨LGG细胞转移和侵袭的机制和治疗策略。通过筛选158个FAM相关基因，并将512个LGG样本聚类为两个亚型（C1和C2），进行差异基因表达分析和功能富集。比较了两种亚型之间的免疫细胞评分和预后，C1 显示较差的结果和较高的免疫评分。在不同的数据集中识别并验证了四基因特征（PHEX、SHANK2、HOPX 和 LGALS1），证明了稳定的预测效果。细胞实验证实了LGALS1和HOPX在促进肿瘤细胞增殖、迁移和侵袭方面的作用，而SHANK2则表现出抑制作用。这种基于 FAM 相关基因的四基因特征为理解 LGG 的发病机制和临床管理提供了宝贵的见解。AJCR 版权所有 © 2024。

Heterogeneity at biological and transcriptomic levels poses a challenge in defining and typing low-grade glioma (LGG), leading to a critical need for specific molecular signatures to enhance diagnosis, therapy, and prognostic evaluation of LGG. This study focused on fatty acid metabolism (FAM) related genes and prognostic features to investigate the mechanisms and treatment strategies for LGG cell metastasis and invasion. By screening 158 FAM-related genes and clustering 512 LGG samples into two subtypes (C1 and C2), differential gene expression analysis and functional enrichment were performed. The immune cell scores and prognosis were compared between the two subtypes, with C1 showing poorer outcomes and higher immune scores. A four-gene signature (PHEX, SHANK2, HOPX, and LGALS1) was identified and validated across different datasets, demonstrating a stable predictive effect. Cellular experiments confirmed the roles of LGALS1 and HOPX in promoting tumor cell proliferation, migration, and invasion, while SHANK2 exhibited a suppressive effect. This four-gene signature based on FAM-related genes offers valuable insights for understanding the pathogenesis and clinical management of LGG.AJCR Copyright © 2024.