Dachsous 钙粘蛋白相关蛋白 1 (DCHS1) 是钙依赖性粘附膜蛋白之一，主要参与哺乳动物组织的发育。目前关于DCHS1在泛癌中的生物学功能缺乏更详细的研究。我们利用TCGA、UALCAN、HPA、GEPIA2等数据库评估了DCHS1的表达、预后价值、诊断价值和基因组变化。 0 和 GSCA。我们利用UCSC、TIMER2.0、TISIDB、GSCA数据库分析DCHS1表达与免疫微环境、干性、TMB、MSI和抗癌药物敏感性之间的关联。使用BioGRID、STRING和GEPIA2.0进行蛋白质相互作用和功能富集分析。通过实时定量PCR、CCK8、Transwell实验和Western blot来确定DCHS1在UCEC中的功能。DCHS1在多种癌症中存在差异表达，其表达与肿瘤的预后和诊断显着相关。在大多数癌症中，DCHS1 表达与癌症相关成纤维细胞 (CAF)、内皮细胞 (EC) 和造血干细胞的浸润显着相关。此外，DCHS1 与许多抗肿瘤药物的敏感性显着相关。功能富集分析显示DCHS1相关蛋白参与粘着斑、子宫内膜癌和Wnt信号通路。 GSEA 结果显示 DCHS1 与许多癌症中的上皮间质转化（EMT）相关。 UCEC 体外实验表明 DCHS1 调节细胞增殖、迁移和 EMT。我们的研究结果表明 DCHS1 可能是一种新型的预后和诊断生物标志物和免疫治疗靶点，并在 UCEC 的增殖、迁移和 EMT 中发挥重要作用。© 2024 . 作者。

Dachsous cadherin related 1 (DCHS1) is one of calcium-dependent adhesion membrane proteins and is mainly involved in the development of mammalian tissues. There is a lack of more detailed research on the biological function of DCHS1 in pan-cancer.We evaluated the expression, the prognostic value, the diagnostic value and genomic alterations of DCHS1 by using the databases, including TCGA, UALCAN, HPA, GEPIA2.0 and GSCA. We employed the databases of UCSC, TIMER2.0, TISIDB, GSCA to analyze the association between DCHS1 expression and the immune microenvironment, stemness, TMB, MSI and anticancer drug sensitivity. BioGRID, STRING and GEPIA2.0 were used to perform protein interaction and functional enrichment analysis. Real-time quantitative PCR, CCK8, Transwell assay and Western blot were performed to determine the function of DCHS1 in UCEC.DCHS1 is differentially expressed in many cancers and its expression is significantly associated with tumor prognosis and diagnosis. DCHS1 expression was significantly correlated with the infiltration of cancer-associated fibroblasts (CAFs), Endothelial cell (ECs), and Hematopoietic stem cell in most cancers. In addition, DCHS1 was significantly associated with sensitivity to many antitumor drugs. Functional enrichment analysis revealed that DCHS1-related proteins were involved in Focal adhesion, Endometrial cancer and Wnt signaling pathway. GSEA results showed that DCHS1 was related to epithelial-mesenchymal transition (EMT) in many cancers. In vitro experiments in UCEC showed that DCHS1 regulated cell proliferation, migration and EMT.Our findings indicated that DCHS1 might be a novel prognostic and diagnostic biomarker and immunotherapy target, and plays an important role in the proliferation, migration and EMT in UCEC.© 2024. The Author(s).