雌激素受体α（ERα）是早期乳腺癌诊断的重要生物标志物。在本研究中，我们提出了一种电化学适体传感器，以纳米材料碳纳米角/金纳米颗粒复合物（1-AP-CNHs/AuNPs）为底物，抗体上的伯胺基团引发单体氨基的开环聚合（ROP）电极表面的酸-二茂铁 (NCA-Fc) 用于超灵敏检测 ERα。 1-AP-CNHs/AuNPs复合材料不仅拥有更多的活性位点，而且增加了电极的比表面积，允许大量二茂铁聚合物长链接枝到电极表面，实现信号放大。在最佳条件下，该方法的检出限为11.995 fg mL-1，检测范围为100 fg mL-1-100 ng mL-1。此外，采用生物素-链霉亲和素系统进一步提高了传感器的灵敏度。重要的是，这种方法可以应用于实际样品中 ERα 的实际检测。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Estrogen receptor alpha (ERα) serves as a crucial biomarker for early breast cancer diagnosis. In this study, we proposed an electrochemical aptasensor with nanomaterial carbon nanohorns/gold nanoparticle composites (1-AP-CNHs/AuNPs) as the substrate, and the primary amine groups on the antibody initiated the ring-opening polymerization (ROP) of monomer amino acid-ferrocene (NCA-Fc) on the electrode surface for ultrasensitive detection of ERα. The composite of 1-AP-CNHs/AuNPs not only possessed more active sites, but also increased the specific surface area of the electrode and allowed a large amount of ferrocene polymer long chains to be grafted onto the electrode surface to achieve signal amplification. Under optimal conditions, the detection limit of the method was 11.995 fg mL-1 with a detection range of 100 fg mL-1-100 ng mL-1. In addition, the biotin-streptavidin system was used to further improve the sensitivity of the sensor. Importantly, this approach could be applied for the practical detection of ERα in real samples.Copyright © 2024 Elsevier B.V. All rights reserved.