黄曲霉毒素B1可以穿透血脑屏障并杀死神经细胞，会污染传统草药，对人类健康构成重大风险。本研究检查了抗骨质疏松药物续断黄曲霉毒素 B1 污染的细胞、认知和行为后果。通过用全反式视黄酸治疗动物，建立了骨质疏松症小鼠模型。然后给动物灌胃续断水煎剂，其中是否含有可检测到的黄曲霉毒素B1。比较动物的骨矿物质密度和矿物盐含量、促炎因子的产生、海马神经发生和小胶质细胞活化、以及行为和认知功能。黄曲霉毒素B1污染续断后，药物含量显着降低。具有生物活性的皂苷。它破坏了草药汤剂提高患病小鼠骨骼中矿物质密度和矿物盐含量的能力，并诱导氧化应激标记物丙二醛以及促炎细胞因子白细胞介素-1β和肿瘤坏死因子的产生。 α。黄曲霉毒素 B1 污染抑制了新神经元的形成，并增加了海马中激活的小胶质细胞的比例。这些神经系统变化与快感缺失、行为绝望以及短期记忆和社会记忆缺陷有关。黄曲霉毒素 B1 污染续断不仅会消除草药煎剂的抗骨质疏松作用，还会引起神经毒性，从而导致认知障碍。衰退和行为异常。应通过严格监管药草的生产和质量控制来避免此类污染。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Aflatoxin B1, which can penetrate the blood-brain barrier and kill neural cells, can contaminate traditional herbal medicines, posing a significant risk to human health. The present study examined cellular, cognitive and behavioral consequences of aflatoxin B1 contamination of the anti-osteoporotic medicine Radix Dipsaci.A mouse model of osteoporosis was created by treating the animals with all-trans-retinoic acid. Then the animals were treated intragastically with water decoctions of Radix Dipsaci that contained detectable aflatoxin B1 or not. The animals were compared in terms of mineral density and mineral salt content of bone, production of pro-inflammatory factors, neurogenesis and microglial activation in hippocampus, as well as behavior and cognitive function.Contamination of Radix Dipsaci with aflatoxin B1 significantly reduced the medicine's content of bioactive saponins. It destroyed the ability of the herbal decoction to improve mineral density and mineral salt content in the bones of diseased mice, and it induced the production of the oxidative stress marker malondialdehyde as well as the pro-inflammatory cytokines interleukin-1β and tumor necrosis factor-α. Aflatoxin B1 contamination inhibited formation of new neurons and increased the proportion of activated microglia in the hippocampus. These neurological changes were associated with anhedonia, behavioral despair, and deficits in short-term memory and social memory.Contamination of Radix Dipsaci with aflatoxin B1 not only eliminates the herbal decoction's anti-osteoporotic effects, but it also induces neurotoxicity that can lead to cognitive decline and behavioral abnormalities. Such contamination should be avoided through tightly regulated production and quality control of medicinal herbs.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.