亚油酸 (LA) 是一种 omega-6 多不饱和脂肪酸。共轭亚油酸 (CLA) 是 LA 异构体家族，包括反式脂肪酸和顺式脂肪酸。这两种脂肪酸都在维持健康方面发挥着营养作用。炎症在包括癌症在内的许多疾病的发病机制中至关重要。本研究发现，LA与CLA的组合（LA/CLA）各自没有作用，但在体外对炎症巨噬细胞RAW264.7细胞有很强的抗协同作用。细胞在含有胎牛血清的 DMEM 中培养，含或不含 LA、CLA 或 LA/CLA 组合。相对较低浓度的LA和CLA的组合物协同抑制细胞生长，导致细胞数量减少。这种效应的根本机制是 Ras、PI3K、Akt、MAPK 和 mTOR 水平降低，以及与细胞生长相关的 p21、p53 和 Rb 水平升高。此外，较低浓度的 LA 和 CLA 组合会刺激与 caspase-3 和裂解 caspase-3 水平增加相关的潜在细胞死亡。值得注意的是，该组合物协同抑制 TNF-α、IL-6 和 PGE2 的产生，这些物质是炎症的主要介质，并刺激 RAW264.7 细胞中的脂多糖。这种效应与 COX-1、COX- 水平降低有关。 2、NF-κB p65。这项研究可能为利用 LA 和 CLA 的组合物治疗炎症提供有用的工具。版权所有 © 2024。由 Elsevier B.V. 出版。

Linoleic acid (LA) is an omega-6 polyunsaturated fatty acid. Conjugated linoleic acid (CLA) is a family of LA isomers that includes both a trans fatty acid and a cis fatty acid. Both fatty acids play a nutritional role in maintaining health. Inflammation is critical in the pathogenesis of many diseases, including cancer. This study found that the combination of LA and CLA (LA/CLA), each of which had no effect, had a strong anti-synergistic effect on inflammatory macrophage RAW264.7 cells in vitro. Cells were cultured in a DMEM containing fetal bovine serum with or without either LA, CLA, or a combination of LA/CLA. The composition of LA and CLA at a comparatively lower concentration synergistically suppressed cell growth, resulting in a reduction in cell number. The underlying mechanism of this effect was based on reduced levels of Ras, PI3K, Akt, MAPK, and mTOR and elevated levels of p21, p53, and Rb, which are associated with cell growth. In addition, the combination of LA and CLA at a lower concentration stimulated potential cell death associated with increased caspase-3 and cleaved caspase-3 levels. Notably, this composition synergistically suppressed the production of TNF-α, IL-6, and PGE2, which are a major mediator of inflammation, with lipopolysaccharide stimulation in RAW264.7 cells This effect was associated with decreased levels of COX-1, COX-2, and NF-κB p65. This study may provide a useful tool for treating inflammatory conditions with the composition of LA and CLA.Copyright © 2024. Published by Elsevier B.V.