本研究旨在以甲氨蝶呤（MTX）为模型抗癌模板，通过沉淀自由基聚合工艺合成核壳明胶基碳量子点分子印迹聚合物（MIP@g-CQD）。为了研究所制备的光致发光MIP@g-CQD作为pH响应纳米载体的效率，通过浸泡在药物溶液中将MTX负载到MIP@g-CQD中，并在必要的条件下评估负载药物的释放行为。 pH 值（7.4、5）。使用 PL、TEM、FE-SEM、DLS 和 FT-IR 分析对材料的成功合成进行了表征。有趣的是，核壳纳米载体中产生的空腔可以与MTX分子有效相互作用，从而增加负载能力。根据Langmuir吸附等温线得到的结果，计算出印迹因子(IF=4.91)。此外，MTX 的结合动力学揭示了核-壳聚合物网络中特定识别位点的创建。负载MTX的MIP@g-CQD在模拟生理环境（pH7.4，37℃）下表现出低速率和有限的释放，但在肿瘤组织（pH5，41℃）条件下释放增加，这可能导致以达到预期目标长期持续释放 MTX。 MIP@g-CQD的这一特性可以避免正常生理条件下MTX的释放，减少MTX药物可能产生的副作用。由于纳米载体结构中酰胺官能团的存在及其带负电荷的性质，MTT测定显示负载MTX的纳米载体对乳腺癌细胞系(MCF-7)具有理想的细胞毒性。根据所获得的结果，所制备的具有适当pH响应性的安全光致发光MIP@g-CQD具有较高的抗癌和生物检测剂应用能力。版权所有©2024。由Elsevier B.V.出版。

This study aims to synthesize a core-shell gelatin-based carbon quantum dot-molecularly imprinted polymer (MIP@g-CQD) via the precipitation free-radical polymerization process using methotrexate (MTX) as a model anticancer template. To investigate the efficiency of the prepared photoluminescent MIP@g-CQD as a pH-responsive nano-carrier, MTX was loaded into MIP@g-CQD by soaking in a drug solution and the release behavior of the loaded drug was evaluated in the necessary pH values (7.4, 5). The successful synthesis of materials was characterized using PL, TEM, FE-SEM, DLS, and FT-IR analyses. Interestingly, the created cavities in the core-shell nano-carriers can interact with the MTX molecules effectively, leading to an increase in the loading capacity. According to the obtained results from Langmuir adsorption isotherms, the imprinting factor was calculated (IF = 4.91). Also, the binding kinetics of MTX revealed the creation of particular recognition sites in the core-shell polymeric network. The MTX-loaded MIP@g-CQD displayed a low rate and limited release at the simulated physiological environment (pH 7.4, 37 °C), but it is increased at tumor tissue (pH 5, 41 °C) conditions, which can lead to long-term and sustained release of MTX in the desired target. This property of MIP@g-CQD could avoid the release of MTX in normal physiological conditions, decreasing the possible side effects of MTX drug. Owing to the existence of amide functional groups in the nano-carrier structure and its negatively charged nature, the MTT assay displayed desirable cytotoxicity against the breast cancer cell line (MCF-7) for the MTX-loaded nano-carrier. According to the obtained results, the prepared safe photoluminescent MIP@g-CQD with appropriate pH-responsivity has a high ability to be applied as an anticancer and bio-detection agent.Copyright © 2024. Published by Elsevier B.V.