通过分级醇沉法获得了六种蛹虫草多糖（命名为CMP-1、CMP-2、CMP-3、CMP-4、CMP-9和CMP-A）。实验结果表明，6种北虫草多糖具有相似的化学组成和光谱特征，但分子量、单糖组成和抗肿瘤活性不同。 CMP-9 的纯化产生小分子多糖LMW-CMP (3.06kDa)。结构实验表明LMW-CMP是一种以(1→4)-α-D-Glcp为主链、O-6位连接有葡萄糖支链的α-葡聚糖。细胞实验结果表明，LMW-CMP能够有效抑制HepG2细胞的生长和增殖，通过MAPK通路激活下游NF-κB信号通路诱导HepG2细胞凋亡，并阻断细胞凋亡于G1期。动物实验表明，LMW-CMP通过改善免疫器官状态，增加体内免疫细胞活性和细胞因子水平，调节淋巴细胞亚群分布，抑制H22荷瘤小鼠肿瘤细胞增殖，具有一定的抑制肿瘤细胞增殖的作用。肿瘤抑制率为 45.70% (200mg/kg)。版权所有 © 2024。Elsevier B.V. 出版

Six Cordyceps militaris polysaccharides (named CMP-1, CMP-2, CMP-3, CMP-4, CMP-9, and CMP-A) were obtained by fractional alcohol precipitation. The experimental results showed that the six Cordyceps militaris polysaccharides had similar chemical composition and spectral features, and different molecular weights, monosaccharide compositions and anti-tumor activities. Purification of CMP-9 yielded the small molecule polysaccharide LMW-CMP (3.06 kDa). Structural experiments showed that LMW-CMP is an α-glucan with (1 → 4)-α-D-Glcp as the main chain and a glucose branched chain attached at the O-6 position. The results of cell experiments showed that LMW-CMP could effectively inhibit the growth and proliferation of HepG2 cells, activate the downstream NF-κB signaling pathway through the MAPK pathway to induce apoptosis of HepG2 cells, and block apoptosis at the G1 phase. Animal experiments showed that LMW-CMP inhibited the proliferation of tumor cells in H22 tumor-bearing mice by improving the state of immune organs, increasing the activity of immune cells and cytokine levels in the body, and regulating the distribution of lymphocyte subpopulations, with a tumor inhibition rate of 45.70 % (200 mg/kg).Copyright © 2024. Published by Elsevier B.V.