毛毛雪莲是一种传统药材。在我们之前的研究中，从 S. laniceps 的花瓣中分离出一种果胶多糖，SLP-4。在本研究中，通过分析 SLP-4 对巨噬细胞（RAW 264.7 细胞）极化的影响来研究 SLP-4 的免疫调节活性。免疫调节活性测定表明，SLP-4能显着增强胞饮和吞噬能力，​​促进细胞毒分子（一氧化氮，SLP-4浓度为800μg/mL时增加6.4倍）和细胞因子（肿瘤细胞）的表达和分泌。原始巨噬细胞中的坏死因子-α和白细胞介素-6分别增加了7.7和11.9倍。可能的机制可归因于通过Toll样受体2和4激活丝裂原激活蛋白激酶和核因子-κB信号通路。此外，SLP-4显着诱导原始巨噬细胞的M1极化，并将巨噬细胞从M2转移到M1。 M1，但对M1巨噬细胞向M2表型的转化影响不大。总体而言，这些结果证明了 SLP-4 作为有吸引力的免疫调节功能补充剂的潜力。版权所有 © 2024。由 Elsevier B.V. 出版。

Saussurea laniceps is a traditional medicinal herb. In our previous study, a pectin polysaccharide, SLP-4, was isolated from the petals of S. laniceps. In this study, the immunomodulatory activity of SLP-4 was studied by analyzing its effects on macrophage (RAW 264.7 cells) polarization. The immunomodulatory activity assays indicated that SLP-4 could significantly enhance the pinocytic and phagocytic capacity and promote the expression and secretion of cytotoxic molecules (nitric oxide, increased by 6.4 times when the SLP-4 concentration was 800 μg/mL) and cytokines (tumor necrosis factor-α and interleukin-6 increased by 7.7 and 11.9 times, respectively) in original macrophage. The possible mechanism could be attributed to the activation of the mitogen-activated protein kinase and nuclear factor-κB signaling pathways through Toll-like receptors 2 and 4. Moreover, SLP-4 significantly induced M1 polarization of original macrophages and transferred macrophages from M2 to M1, but had little effect on the conversion of M1 macrophages into M2 phenotype. Overall, these results demonstrate the potential of SLP-4 as an attractive immunomodulating functional supplement.Copyright © 2024. Published by Elsevier B.V.