前列腺癌作为一个重要的全球健康问题，需要探索新的治疗方法。诺斯卡品是一种鸦片衍生的苯酞异喹啉生物碱，由于其抗肿瘤特性，在癌症治疗中显示出了前景。然而，生物利用度低和潜在副作用等限制阻碍了其临床应用。本研究引入 Nanonoscapine 作为一种新型药物来克服这些挑战，利用纳米技术改善药物输送和疗效的优势。我们监测了 nanonoscapine 对雄激素敏感的人前列腺癌细胞系 LNCaP 的影响，研究了其对 GLI1 和 BAX 基因表达（细胞周期和细胞凋亡的关键调节因子）的影响。我们的 MTT 测定、流式细胞术和基因表达分析结果表明，nanoscapine 通过诱导 G2/M 期阻滞和细胞凋亡，有效抑制前列腺癌细胞增殖。此外，通过生物信息学和计算分析，我们揭示了潜在的分子机制，强调了 nanonoscapine 在改善患者预后方面的治疗潜力。这项研究强调了 nanonoscapine 作为传统化疗的替代或辅助治疗的重要性，值得在临床环境中进行进一步研究。© 2024。作者。

Prostate cancer as a critical global health issue, requires the exploration of a novel therapeutic approach. Noscapine, an opium-derived phthalide isoquinoline alkaloid, has shown promise in cancer treatment thanks to its anti-tumorigenic properties. However, limitations such as low bioavailability and potential side effects have hindered its clinical application. This study introduces nanonoscapine as a novel medication to overcome these challenges, leveraging the advantages of improved drug delivery and efficacy achieved in nanotechnology. We monitored the effects of nanonoscapine on the androgen-sensitive human prostate adenocarcinoma cell line, LNCaP, investigating its impact on GLI1 and BAX genes' expressions, crucial regulators of cell cycle and apoptosis. Our findings, from MTT assays, flow cytometry, and gene expression analyses, have demonstrated that nanonoscapine effectively inhibits prostate cancer cell proliferation by inducing G2/M phase arrest and apoptosis. Furthermore, through bioinformatics and computational analyses, we have revealed the underlying molecular mechanisms, underscoring the therapeutic potential of nanonoscapine in enhancing patient outcomes. This study highlights the significance of nanonoscapine as an alternative or adjunct treatment to conventional chemotherapy, warranting further investigation in clinical settings.© 2024. The Author(s).