实体瘤的细胞外基质 (ECM) 以多种方式影响 CD8 T 细胞和自然杀伤 (NK) 细胞的抗肿瘤活性。细胞运动受到肿瘤 ECM 的限制，从而产生物理屏障。肿瘤ECM直接改变细胞毒性淋巴细胞的表型和功能，并间接影响细胞毒性淋巴细胞和癌细胞之间免疫突触介导的相互作用。因此，应该通过考虑细胞毒性淋巴细胞、癌细胞和肿瘤 ECM 之间复杂的三元相互作用来制定改善实体瘤免疫治疗的策略。近似肿瘤 ECM 关键特征的新型生物工程工具，例如体外重构 3D ECM 和微流体，从基础研究角度和转化角度来看都很有价值，旨在实现系统筛选方法。版权所有 © 2024 Elsevier Ltd。保留所有权利。

The extracellular matrix (ECM) of solid tumors impacts the antitumor activities of CD8+ T and natural killer (NK) cells in a variety of ways. Cell motility is restricted by the tumor ECM which creates physical barriers. The tumor ECM directly alter the phenotypes and functions of cytotoxic lymphocytes, and indirectly influences immunological synapse-mediated interactions between cytotoxic lymphocytes and cancer cells. Therefore, strategies to improve solid tumor immunotherapy should be established by considering complex ternary interactions between cytotoxic lymphocytes, cancer cells, and the tumor ECM. Novel bioengineering tools approximating key characteristics of the tumor ECM, such as in vitro reconstituted 3D ECMs and microfluidics are valuable from a fundamental study viewpoint and from a translational perspective, aiming to enable systematic screening approaches.Copyright © 2024 Elsevier Ltd. All rights reserved.