最近的研究表明肠道微生物群可能与脑肿瘤的发生有关，但缺乏直接证据。本研究采用孟德尔随机化研究（MR）方法探讨肠道微生物群与脑肿瘤之间的潜在因果关系。我们利用现有最大的肠道微生物群 Genome-Wide 分析了 211 个肠道微生物群与脑肿瘤之间的全基因组关联数据。关联研究荟萃分析数据 (n=13266) 并将其与 IEU OpenGWAS 数据库中的脑肿瘤数据相结合。我们使用逆方差加权分析，辅以孟德尔随机化-Egger 回归、加权中值估计、简单模式和加权模式等方法来评估因果关系。此外，我们还进行了孟德尔随机化-Egger截距检验、Cochran's Q检验和孟德尔随机化Steiger方向性检验，以保证分析的准确性。质量控制包括敏感性分析、水平基因多效性检验、异质性检验和MR Steiger方向性检验。我们的研究发现特定的肠道微生物类群，如乳杆菌目和梭状芽孢杆菌科1与脑肿瘤的发生呈正相关，而Defluviitaleaceae属则与脑肿瘤的发生呈正相关。 UCG011和Flavonifractor属与脑肿瘤的发生呈负相关。孟德尔随机-Egger截距检验表明我们的分析不受多效性影响（P>0.05）。该研究首次揭示了肠道菌群与脑肿瘤之间的潜在因果关系，为脑肿瘤的预防和治疗提供了新的视角。早期脑肿瘤。这些发现可能有助于制定新的临床干预策略，并为未来的研究指明方向。版权所有 © 2024 Yang、Lu、Dong、Wei、Christian、Huang、Kuang 和 Cao。

Recent studies have shown that gut microbiota may be related to the occurrence of brain tumors, but direct evidence is lacking. This study used the Mendelian randomization study (MR) method to explore the potential causal link between gut microbiota and brain tumors.We analyzed the genome-wide association data between 211 gut microbiota taxa and brain tumors, using the largest existing gut microbiota Genome-Wide Association Studies meta-analysis data (n=13266) and combining it with brain tumor data in the IEU OpenGWAS database. We use inverse-variance weighted analysis, supplemented by methods such as Mendelian randomization-Egger regression, weighted median estimator, simple mode, and weighted mode, to assess causality. In addition, we also conducted the Mendelian randomization-Egger intercept test, Cochran's Q test, and Mendelian randomization Steiger directionality test to ensure the accuracy of the analysis. Quality control includes sensitivity analysis, horizontal gene pleiotropy test, heterogeneity test, and MR Steiger directionality test.Our study found that specific gut microbial taxa, such as order Lactobacillales and family Clostridiaceae1, were positively correlated with the occurrence of brain tumors, while genus Defluviitaleaceae UCG011 and genus Flavonifractor were negatively correlated with the occurrence of brain tumors. The Mendelian randomization-Egger intercept test showed that our analysis was not affected by pleiotropy (P>0.05).This study reveals for the first time the potential causal relationship between gut microbiota and brain tumors, providing a new perspective for the prevention and treatment of early brain tumors. These findings may help develop new clinical intervention strategies and point the way for future research.Copyright © 2024 Yang, Lu, Dong, Wei, Christian, Huang, Kuang and Cao.