近年来，生物材料细胞膜的表面工程引起了各种生物医学应用的极大关注。在这项研究中，我们研究了使用厚度约为 1 μm 的藻酸盐和明胶水凝胶鞘调节细胞周期进程的可能性。通过固定在细胞表面的辣根过氧化物酶催化的交联，在细胞表面形成水凝胶鞘。水凝胶鞘不会降低表达基于荧光泛素化的细胞周期指标 2 (HeLa/Fucci2) 的人宫颈癌细胞系 (HeLa) 的活力（培养 2 天期间 >95%）。用水凝胶鞘包被 HeLa/Fucci2 细胞导致细胞周期停滞在 G2/M 期，这可能是由于 F-肌动蛋白形成减少所致。由于细胞周期停滞，在 HeLa/Fucci2 细胞中观察到细胞生长受到抑制。综上所述，我们的结果表明细胞表面的水凝胶鞘涂层是调节细胞周期进程的可行方法。

Recently, surface engineering of the cell membrane with biomaterials has attracted great attention for various biomedical applications. In this study, we investigated the possibility of modulating cell cycle progression using alginate and gelatin-based hydrogel sheaths with a thickness of ∼1 μm. The hydrogel sheath was formed on cell surfaces through cross-linking catalyzed by horseradish peroxidase immobilized on the cell surface. The hydrogel sheath did not decrease the viability (>95% during 2 days of culture) of the human cervical carcinoma cell line (HeLa) expressing the fluorescent ubiquitination-based cell cycle indicator 2 (HeLa/Fucci2). Coating the HeLa/Fucci2 cells with the hydrogel sheath resulted in a cell cycle arrest in the G2/M phase, which can be caused by the reduced F-actin formation. As a result of this cell cycle arrest, an inhibition of cell growth was observed in the HeLa/Fucci2 cells. Taken together, our results demonstrate that the hydrogel sheath coating on the cell surface is a feasible approach to modulating cell cycle progression.